Compositions and methods for the therapy and diagnosis of prostate cancer Number:6,800,746 from the United States Patent and Trademark Office (PTO) owispatent Compositions and methods for the therapy and diagnosis of cancer, particularly prostate cancer, are disclosed. Illustrative compositions comprise one or more prostate-specific polypeptides, immunogenic portions thereof, polynucleotides that encode such polypeptides, antigen presenting cell that expresses such polypeptides, and T cells that are specific for cells expressing such polypeptides. The disclosed compositions are useful, for example, in the diagnosis, prevention and/or treatment of diseases, particularly prostate cancer.<H Compositions, diagnosis, Compositions, an, 6800746.html, Patent, pto, 6800746

Title: Compositions and methods for the therapy and diagnosis of prostate cancer

Abstract: Compositions and methods for the therapy and diagnosis of cancer, particularly prostate cancer, are disclosed. Illustrative compositions comprise one or more prostate-specific polypeptides, immunogenic portions thereof, polynucleotides that encode such polypeptides, antigen presenting cell that expresses such polypeptides, and T cells that are specific for cells expressing such polypeptides. The disclosed compositions are useful, for example, in the diagnosis, prevention and/or treatment of diseases, particularly prostate cancer.

Patent Number: 6,800,746 Issued on 10/05/2004 to Xu, et al.

Inventors: Xu; Jiangchun (Bellevue, WA), Dillon; Davin C. (Issaquah, WA), Mitcham; Jennifer L. (Redmond, WA), Harlocker; Susan L. (Seattle, WA), Jiang; Yuqiu (Kent, WA), Kalos; Michael D. (Seattle, WA), Fanger; Gary R. (Mill Creek, WA), Retter; Marc W. (Carnation, WA), Stolk; John A. (Bothell, WA), Day; Craig H. (Seattle, WA), Vedvick; Thomas S. (Federal Way, WA), Carter; Darrick (Seattle, WA), Li; Samuel X. (Redmond, WA), Wang; Aijun (Issaquah, WA), Skeiky; Yasir A. W. (Bellevue, WA), Hepler; William T. (Seattle, WA), Henderson; Robert A. (Edmonds, WA)
Assignee: Corixa Corporation (Seattle, WA)

Appl. No.: 09/759,143

Filed: January 12, 2001

Related U.S. Patent Documents


Application Number	Filing Date	Patent Number	Issue Date
685166	Oct., 2000	6630305
679426	Oct., 2000
657279	Sep., 2000
651236	Aug., 2000
636215	Aug., 2000	6620922
605783	Jun., 2000
593793	Jun., 2000
570737	May., 2000
568100	May., 2000
536857	Mar., 2000
483672	Jan., 2000
443686	Nov., 1999
439313	Nov., 1999	6329505
352616	Jul., 1999	6395278
288946	Apr., 1999
232149	Jan., 1999	6465611
159812	Sep., 1998	6613872
115453	Jul., 1998	6657056
030607	Feb., 1998	6262245
020956	Feb., 1998	6261562
904804	Aug., 1997
806099	Feb., 1997

Current U.S. Class: 536/23.1 ; 435/320.1; 435/325; 435/6; 536/24.1

Field of Search: 435/6,250.3,320.1,325 536/23.1,24.1

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Foreign Patent Documents


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WO 96/21671	Jul., 1996	WO
WO 97/33909	Sep., 1997	WO
WO 98/12302	Mar., 1998	WO
WO 98/17687	Apr., 1998	WO
WO 98/20117	May., 1998	WO
WO 98/31799	Jul., 1998	WO
WO 98/37093	Aug., 1998	WO
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WO 98/38310	Sep., 1998	WO
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Primary Examiner: Brusca; John S.
Assistant Examiner: Zhou; Shubo Joe
Attorney, Agent or Firm: Seed IP Law Group PLLC

Parent Case Text

CROSS REFERENCE TO RELATED APPLICATIONS

This application is continuation-in-part of U.S. patent application Ser. No. 09/685,166, filed Oct. 10, 2000 now U.S. Pat. No. 6,630,305, U.S. patent application Ser. No. 09/679,426, filed Oct. 2, 2000, U.S. patent application Ser. No. 09/657,279, filed Sep. 6, 2000; U.S. application Ser. No. 09/651,236, filed Aug. 29, 2000; U.S. application Ser. No. 09/636,215, filed Aug. 9, 2000 now U.S. Pat. No. 6,620,972; U.S. application Ser. No. 09/605,783, filed Jun. 27, 2000; U.S. application Ser. No. 09/593,793, filed Jun. 13, 2000; U.S. application Ser. No. 09/570,737, filed May 12, 2000; U.S. application Ser. No. 09/568,100, filed May 9, 2000; U.S. application Ser. No. 09/536,857, filed Mar. 27, 2000 now abandoned; U.S. application Ser. No. 09/483,672, filed Jan. 14, 2000; U.S. application Ser. No. 09/443,686, filed Nov. 18, 1999 now abandoned; U.S. application Ser. No. 09/439,313, filed Nov. 12, 1999 now U.S. Pat. No. 6,329,505; U.S. application Ser. No. 09/352,616, filed Jul. 13, 1999 now U.S Pat. No. 6,395,278; U.S. application Ser. No. 09/288,946, filed Apr. 9, 1999 now abandoned; U.S. application Ser. No. 09/232,149, filed Jan. 15, 1999 now U.S Pat. No. 6,465,611; U.S. application Ser. No. 09/159,812, filed Sep. 23, 1998 now U.S Pat. No. 6,613,872; U.S. application Ser. No. 09/115,453, filed Jul. 14, 1998 now U.S. Pat. No. 6,657,056; U.S. application Ser. No. 09/030,607, filed Feb. 25, 1998 now U.S. Pat. No. 6,262,245; U.S. application Ser. No. 09/020,956, filed Feb. 9, 1998 now U.S. Pat. No. 6,261,562; U.S. application Ser. No. 08/904,804, filed Aug. 1, 1997 (abandoned); U.S. application Ser. No. 08/806,099, filed Feb. 25, 1997 (abandoned); each a CIP of the previously filed application and pending unless noted.

Claims

What is claimed:

1. An isolated polynucleotide encoding at least an immunogenic portion of the polypeptide encoded by SEQ ID NO: 110, wherein the immunogenic portion is selected from the group consisting of amino acid residues 106-553, 136-547, or 1-472 of the polypeptide encoded by SEQ ID NO: 110.

2. An isolated polynucleotide encoding at least an immunogenic portion of the polypeptide encoded by SEQ ID NO: 110, wherein the immunogenic portion is selected from the group consisting of amino acid residues 1-325, 58-76, 39-57, or 1-19 of the polypeptide encoded by SEQ ID NO: 110.

3. An isolated polynucleotide encoding at least an immunogenic portion of the polypeptide encoded by SEQ ID NO: 110, wherein the immunogenic portion is selected from the group consisting of amino acid residues 181-198, or 296-322 of the polypeptide encoded by SEQ ID NO: 110.

4. A recombinant vector comprising a polynucleotide of any one of claims 1, 2 and 3.

5. An expression vector comprising a polynucleotide of any one of claims 1, 2 and 3, operably linked to an expression control sequence.

6. A host cell transformed or transfected with an expression vector according to claim 5.

7. An expression vector according to claim 5, wherein the vector is a viral vector.

8. An expression vector according to claim 5, wherein the vector is a viral vector selected from the group consisting of an adenovirus vector, adeno-associated virus vector, semliki forest virus vector, sindbis virus vector, pox virus vector, vaccina virus vector, avian poxvirus vector, avipox virus vector, fowlpox virus vector, canarypox virus vector, alphavirus vector, fowlpox virus vector or lentivirus vector.

9. A composition comprising a first component selected from the group consisting of physiologically acceptable carriers and immunostimulants, and a second component selected from the group consisting of a polynucleotide according to any one of claims 1, 2, and 3 or an expression vector comprising a polynucleotide according to any one of claims 1, 2, and 3.

Description

TECHNICAL FIELD OF THE INVENTION

The present invention relates generally to therapy and diagnosis of cancer, such as prostate cancer. The invention is more specifically related to polypeptides, comprising at least a portion of a prostate-specific protein, and to polynucleotides encoding such polypeptides. Such polypeptides and polynucleotides are useful in pharmaceutical compositions, e.g., vaccines, and other compositions for the diagnosis and treatment of prostate cancer.

BACKGROUND OF THE INVENTION

Cancer is a significant health problem throughout the world. Although Cancer is a significant health problem throughout the world. Although advances have been made in detection and therapy of cancer, no vaccine or other universally successful method for prevention or treatment is currently available. Current therapies, which are generally based on a combination of chemotherapy or surgery and radiation, continue to prove inadequate in many patients.

Prostate cancer is the most common form of cancer among males, with an estimated incidence of 30% in men over the age of 50. Overwhelming clinical evidence shows that human prostate cancer has the propensity to metastasize to bone, and the disease appears to progress inevitably from androgen dependent to androgen refractory status, leading to increased patient mortality. This prevalent disease is currently the second leading cause of cancer death among men in the U.S.

In spite of considerable research into therapies for the disease, prostate cancer remains difficult to treat. Commonly, treatment is based on surgery and/or radiation therapy, but these methods are ineffective in a significant percentage of cases. Two previously identified prostate specific proteins--prostate specific antigen (PSA) and prostatic acid phosphatase (PAP)--have limited therapeutic and diagnostic potential. For example, PSA levels do not always correlate well with the presence of prostate cancer, being positive in a percentage of non-prostate cancer cases, including benign prostatic hyperplasia (BPH). Furthermore, PSA measurements correlate with prostate volume, and do not indicate the level of metastasis.

In spite of considerable research into therapies for these and other cancers, prostate cancer remains difficult to diagnose and treat effectively. Accordingly, there is a need in the art for improved methods for detecting and treating such cancers. The present invention fulfills these needs and further provides other related advantages.

SUMMARY OF THE INVENTION

In one aspect, the present invention provides polynucleotide compositions comprising a sequence selected from the group consisting of: (a) sequences provided in SEQ ID NO: 1-111, 115-171, 173-175, 177, 179-305, 307-315, 326, 328, 330, 332-335, 340-375, 381, 382 and 384-476, 524, 526, 530, 531, 533, 535, 536, 552, 569-572, 587, 591, 593-606, 618-705, 709-774, 777, 789, 817, 823, 824, 878, 880-882, 894, 896, 907, 908, 916-919 and 929-931; (b) complements of the sequences provided in SEQ ID NO: 1-111, 115-171, 173-175, 177, 179-305, 307-315, 326, 328, 330, 332-335, 340-375, 381, 382 and 384-476, 524, 526, 530, 531, 533, 535, 536, 552, 569-572, 587, 591, 593-606, 618-705, 709-774, 777, 789, 817, 823, 824, 878, 880-882, 894, 896, 907, 908, 916-919 and 929-931; (c) sequences consisting of at least 20 contiguous residues of a sequence provided in SEQ ID NO: 1-111, 115-171, 173-175, 177, 179-305, 307-315, 326, 328, 330, 332-335, 340-375, 381, 382 and 384-476, 524, 526, 530, 531, 533, 535, 536, 552, 569-572, 587, 591, 593-606, 618-705, 709-774, 777, 789, 817, 823, 824, 878, 880-882, 894, 896, 907, 908, 916-919 and 929-931; (d) sequences that hybridize to a sequence provided in SEQ ID NO: 1-111, 115-171, 173-175, 177, 179-305, 307-315, 326, 328, 330, 332-335, 340-375, 381, 382 and 384-476, 524, 526, 530, 531, 533, 535, 536, 552, 569-572, 587, 591, 593-606, 618-705, 709-774, 777, 789, 817, 823, 824, 878, 880-882, 894, 896, 907, 908, 916-919 and 929-931, under moderately stringent conditions; (e) sequences having at least 75% identity to a sequence of SEQ ID NO: 1-111, 115-171, 173-175, 177, 179-305, 307-315, 326, 328, 330, 332-335, 340-375, 381, 382 and 384-476, 524, 526, 530, 531, 533, 535, 536, 552, 569-572, 587, 591, 593-606, 618-705, 709-774, 777, 789, 817, 823, 824, 878, 880-882, 894, 896, 907, 908, 916-919 and 929-931; (f) sequences having at least 90% identity to a sequence of SEQ ID NO: 1-111, 115-171, 173-175, 177, 179-305, 307-315, 326, 328, 330, 332-335, 340-375, 381, 382 and 384-476, 524, 526, 530, 531, 533, 535, 536, 552, 569-572, 587, 591, 593-606, 618-705, 709-774, 777, 789, 817, 823, 824, 878, 880-882, 894, 896, 907, 908, 916-919 and 929-931; and (g) degenerate variants of a sequence provided in SEQ ID NO: 1-111, 115-171, 173-175, 177, 179-305, 307-315, 326, 328, 330, 332-335, 340-375, 381, 382 and 384-476, 524, 526, 530, 531, 533, 535, 536, 552, 569-572, 587, 591, 593-606, 618-705, 709-774, 777, 789, 817, 823, 824, 878, 880-882, 894, 896, 907, 908, 916-919 and 929-931.

In one preferred embodiment, the polynucleotide compositions of the invention are expressed in at least about 20%, more preferably in at least about 30%, and most preferably in at least about 50% of prostate tissue samples tested, at a level that is at least about 2-fold, preferably at least about 5-fold, and most preferably at least about 10-fold higher than that for other normal tissues.

The present invention, in another aspect, provides polypeptide compositions comprising an amino acid sequence that is encoded by a polynucleotide sequence described above.

The present invention further provides polypeptide compositions comprising an amino acid sequence selected from the group consisting of sequences recited in SEQ ID NO: 112-114, 172, 176, 178, 327, 329, 331, 336, 339, 376-380, 383, 477-483, 496, 504, 505, 519, 520, 522, 525, 527, 532, 534, 537-551, 553-568, 573-586, 588-590, 592, 706-708, 775, 776, 778, 780, 781, 811, 814, 818, 826, 827, 853, 855, 858, 860-862, 866-877, 879, 883-893, 895, 897, 898, 909-915, 920-928 and 932-934.

In certain preferred embodiments, the polypeptides and/or polynucleotides of the present invention are immunogenic, i.e., they are capable of eliciting an immune response, particularly a humoral and/or cellular immune response, as further described herein.

The present invention further provides fragments, variants and/or derivatives of the disclosed polypeptide and/or polynucleotide sequences, wherein the fragments, variants and/or derivatives preferably have a level of immunogenic activity of at least about 50%, preferably at least about 70% and more preferably at least about 90% of the level of immunogenic activity of a polypeptide sequence set forth in SEQ ID NO: 112-114, 172, 176, 178, 327, 329, 331, 336, 339, 376-380, 383, 477-483, 496, 504, 505, 519, 520, 522, 525, 527, 532, 534, 537-551, 553-568, 573-586, 588-590, 592, 706-708, 775, 776, 778, 780, 781, 811, 814, 818, 826, 827, 853, 855, 858 or 860-862, or a polypeptide sequence encoded by a polynucleotide sequence set forth in SEQ ID NO: 1-111, 115-171, 173-175, 177, 179-305, 307-315, 326, 328, 330, 332-335, 340-375, 381, 382 and 384-476, 524, 526, 530, 531, 533, 535, 536, 552, 569-572, 587, 591, 593-606, 618-705, 709-774, 777, 789, 817, 823, 824, 878, 880-882, 894, 896, 907, 908, 916-919 and 929-931.

The present invention further provides polynucleotides that encode a polypeptide described above, expression vectors comprising such polynucleotides and host cells transformed or transfected with such expression vectors.

Within other aspects, the present invention provides pharmaceutical compositions comprising a polypeptide or polynucleotide as described above and a physiologically acceptable carrier.

Within a related aspect of the present invention, pharmaceutical compositions, e.g., vaccine compositions, are provided for prophylactic or therapeutic applications. Such compositions generally comprise an immunogenic polypeptide or polynucleotide of the invention and an immunostimulant, such as an adjuvant, together with a physiologically acceptable carrier.

The present invention further provides pharmaceutical compositions that comprise: (a) an antibody or antigen-binding fragment thereof that specifically binds to a polypeptide of the present invention, or a fragment thereof, and (b) a physiologically acceptable carrier.

Within further aspects, the present invention provides pharmaceutical compositions comprising: (a) an antigen presenting cell that expresses a polypeptide as described above and (b) a pharmaceutically acceptable carrier or excipient. Illustrative antigen presenting cells include dendritic cells, macrophages, monocytes, fibroblasts and B cells.

Within related aspects, pharmaceutical compositions are provided that comprise: (a) an antigen presenting cell that expresses a polypeptide as described above and (b) an immunostimulant.

The present invention further provides, in other aspects, fusion proteins that comprise at least one polypeptide as described above, as well as polynucleotides encoding such fusion proteins, typically in the form of pharmaceutical compositions, e.g., vaccine compositions, comprising a physiologically acceptable carrier and/or an immunostimulant. The fusions proteins may comprise multiple immunogenic polypeptides or portions/variants thereof, as described herein, and may further comprise one or more polypeptide segments for facilitating and/or enhancing the expression, purification and/or immunogenicity of the polypeptide(s).

Within further aspects, the present invention provides methods for stimulating an immune response in a patient, preferably a T cell response in a human patient, comprising administering a pharmaceutical composition described herein. The patient may be afflicted with prostate cancer, in which case the methods provide treatment for the disease, or a patient considered to be at risk for such a disease may be treated prophylactically.

Within further aspects, the present invention provides methods for inhibiting the development of a cancer in a patient, comprising administering to a patient a pharmaceutical composition as recited above. The patient may be afflicted with prostate cancer, in which case the methods provide treatment for the disease, or a patient considered to be at risk for such a disease may be treated prophylactically.

The present invention further provides, within other aspects, methods for removing tumor cells from a biological sample, comprising contacting a biological sample with T cells that specifically react with a polypeptide of the present invention, wherein the step of contacting is performed under conditions and for a time sufficient to permit the removal of cells expressing the polypeptide from the sample.

Within related aspects, methods are provided for inhibiting the development of a cancer in a patient, comprising administering to a patient a biological sample treated as described above.

Methods are further provided, within other aspects, for stimulating and/or expanding T cells specific for a polypeptide of the present invention, comprising contacting T cells with one or more of: (i) a polypeptide as described above; (ii) a polynucleotide encoding such a polypeptide; and (iii) an antigen presenting cell that expresses such a polypeptide; under conditions and for a time sufficient to permit the stimulation and/or expansion of T cells. Isolated T cell populations comprising T cells prepared as described above are also provided.

Within further aspects, the present invention provides methods for inhibiting the development of a cancer in a patient, comprising administering to a patient an effective amount of a T cell population as described above.

The present invention further provides methods for inhibiting the development of a cancer in a patient, comprising the steps of: (a) incubating CD4.sup.+ and/or CD8.sup.+ T cells isolated from a patient with one or more of: (i) a polypeptide comprising at least an immunogenic portion of polypeptide disclosed herein; (ii) a polynucleotide encoding such a polypeptide; and (iii) an antigen-presenting cell that expressed such a polypeptide; and (b) administering to the patient an effective amount of the proliferated T cells, thereby inhibiting the development of a cancer in the patient. Proliferated cells may, but need not, be cloned prior to administration to the patient.

Within further aspects, the present invention provides methods for determining the presence or absence of a cancer, preferably a prostate cancer, in a patient comprising: (a) contacting a biological sample obtained from a patient with a binding agent that binds to a polypeptide as recited above; (b) detecting in the sample an amount of polypeptide that binds to the binding agent; and (c) comparing the amount of polypeptide with a predetermined cut-off value, and therefrom determining the presence or absence of a cancer in the patient. Within preferred embodiments, the binding agent is an antibody, more preferably a monoclonal antibody.

The present invention also provides, within other aspects, methods for monitoring the progression of a cancer in a patient. Such methods comprise the steps of: (a) contacting a biological sample obtained from a patient at a first point in time with a binding agent that binds to a polypeptide as recited above; (b) detecting in the sample an amount of polypeptide that binds to the binding agent; (c) repeating steps (a) and (b) using a biological sample obtained from the patient at a subsequent point in time; and (d) comparing the amount of polypeptide detected in step (c) with the amount detected in step (b), and therefrom monitoring the progression of the cancer in the patient.

The present invention further provides, within other aspects, methods for determining the presence or absence of a cancer in a patient, comprising the steps of: (a) contacting a biological sample obtained from a patient with an oligonucleotide that hybridizes to a polynucleotide of the present invention; (b) detecting in the sample a level of a polynucleotide, preferably mRNA, that hybridizes to the oligonucleotide; and (c) comparing the level of polynucleotide that hybridizes to the oligonucleotide with a predetermined cut-off value, and therefrom determining the presence or absence of a cancer in the patient. Within certain embodiments, the amount of mRNA is detected via polymerase chain reaction using, for example, at least one oligonucleotide primer that hybridizes to a polynucleotide of the present invention, or a complement of such a polynucleotide. Within other embodiments, the amount of mRNA is detected using a hybridization technique, employing an oligonucleotide probe that hybridizes to an inventive polynucleotide, or a complement of such a polynucleotide.

In related aspects, methods are provided for monitoring the progression of a cancer in a patient, comprising the steps of: (a) contacting a biological sample obtained from a patient with an oligonucleotide that hybridizes to a polynucleotide of the present invention; (b) detecting in the sample an amount of a polynucleotide that hybridizes to the oligonucleotide; (c) repeating steps (a) and (b) using a biological sample obtained from the patient at a subsequent point in time; and (d) comparing the amount of polynucleotide detected in step (c) with the amount detected in step (b), and therefrom monitoring the progression of the cancer in the patient.

Within further aspects, the present invention provides antibodies, such as monoclonal antibodies, that bind to a polypeptide as described above, as well as diagnostic kits comprising such antibodies. Diagnostic kits comprising one or more oligonucleotide probes or primers as described above are also provided.

These and other aspects of the present invention will become apparent upon reference to the following detailed description and attached drawings. All references disclosed herein are hereby incorporated by reference in their entirety as if each was incorporated individually.

BRIEF DESCRIPTION OF THE DRAWINGS AND SEQUENCE IDENTIFIERS

FIG. 1 illustrates the ability of T cells to kill fibroblasts expressing the representative prostate-specific polypeptide P502S, as compared to control fibroblasts. The percentage lysis is shown as a series of effector:target ratios, as indicated.

FIGS. 2A and 2B illustrate the ability of T cells to recognize cells expressing the representative prostate-specific polypeptide P502S. In each case, the number of .gamma.-interferon spots is shown for different numbers of responders. In FIG. 2A, data is presented for fibroblasts pulsed with the P2S-12 peptide, as compared to fibroblasts pulsed with a control E75 peptide. In FIG. 2B, data is presented for fibroblasts expressing P502S, as compared to fibroblasts expressing HER-2/neu.

FIG. 3 represents a peptide competition binding assay showing that the P1S#10 peptide, derived from P501S, binds HLA-A2. Peptide P1S#10 inhibits HLA-A2 restricted presentation of fluM58 peptide to CTL clone D150M58 in TNF release bioassay. D150M58 CTL is specific for the HLA-A2 binding influenza matrix peptide fluM58.

FIG. 4 illustrates the ability of T cell lines generated from P1S#10 immunized mice to specifically lyse P1S#10-pulsed Jurkat A2Kb targets and P501S-transduced Jurkat A2Kb targets, as compared to EGFP-transduced Jurkat A2Kb. The percent lysis is shown as a series of effector to target ratios, as indicated.

FIG. 5 illustrates the ability of a T cell clone to recognize and specifically lyse Jurkat A2Kb cells expressing the representative prostate-specific polypeptide P501S, thereby demonstrating that the P1S#10 peptide may be a naturally processed epitope of the P501S polypeptide.

FIGS. 6A and 6B are graphs illustrating the specificity of a CD8.sup.+ cell line (3A-1) for a representative prostate-specific antigen (P501S). FIG. 6A shows the results of a .sup.51 Cr release assay. The percent specific lysis is shown as a series of effector:target ratios, as indicated. FIG. 6B shows the production of interferon-gamma by 3A-1 cells stimulated with autologous B-LCL transduced with P501S, at varying effector:target rations as indicated.

FIG. 7 is a Western blot showing the expression of P501S in baculovirus.

FIG. 8 illustrates the results of epitope mapping studies on P501S. The peptides used in the study are shown from left to right at the bottom of the figure, as follows: MDRLVQRPGTRAVYLASVA (SEQ ID NO: 489), YLASVAAFPVAAGATCLSHS (SEQ ID NO: 490), TCLSHSVAVVTASAALTGFT (SEQ ID NO: 491), ALTGFTFSALQILPYTLASL (SEQ ID NO: 492), YTLASLYHREKQVFLPKYRG (SEQ ID NO: 493), LPKYRGDTGGASSEDSLMIS (SEQ ID NO: 494), DSLMTSFLPGPKPGAPFPNG (SEQ ID NO: 495), APFPNGHVGAGGSGLLPPPPA (SEQ ID NO: 496), LLPPPPALCGASACDVSVRV (SEQ ID NO: 497), DVSVRVVVGEPTEARVVPGR (SEQ ID NO: 498), RVVPGRGICLDLAILDSAFL (SEQ ID NO: 499), LDSAFLLSQVAPSLFMGSIV (SEQ ID NO: 500), FMGSIVQLSQSVTAYMVSAA (SEQ ID NO: 501).

FIG. 9 is a schematic representation of the P501S protein (SEQ ID NO: 113) showing the location of transmembrane domains and predicted intracellular and extracellular domains.

FIG. 10 is a genomic map showing the location of the prostate genes P775P, P704P, B305D, P712P and P774P within the Cat Eye Syndrome region of chromosome 22q11.2

FIG. 11 shows the results of an ELISA assay to determine the specificity of rabbit polyclonal antisera raised against P501S. The depicted sequence corresponding to peptide P501S 306-320 is set forth in SEQ ID NO: 519 and the sequence corresponding to P501S 296-320 is set forth in SEQ ID NO: 520.

FIGS. 12A(1), 12A(2), 12A(3), and B are the full-length cDNA (SEQ ID NO:591) and predicted amino acid (SEQ ID NO:592) sequences, respectively, for the clone P788P. SEQ ID NO: 1 is the determined cDNA sequence for F1-13 SEQ ID NO: 2 is the determined 3' cDNA sequence for F1-12 SEQ ID NO: 3 is the determined 5' cDNA sequence for F1-12 SEQ ID NO: 4 is the determined 3' cDNA sequence for F1-16 SEQ ID NO: 5 is the determined 3' cDNA sequence for H1-1 SEQ ID NO: 6 is the determined 3' cDNA sequence for H1-9 SEQ ID NO: 7 is the determined 3' cDNA sequence for H1-4 SEQ ID NO: 8 is the determined 3' cDNA sequence for J1-17 SEQ ID NO: 9 is the determined 5' cDNA sequence for J1-17 SEQ ID NO: 10 is the determined 3' cDNA sequence for L1-12 SEQ ID NO: 11 is the determined 5' cDNA sequence for L1-12 SEQ ID NO: 12 is the determined 3' cDNA sequence for N1-1862 SEQ ID NO: 13 is the determined 5' cDNA sequence for N1-1862 SEQ ID NO: 14 is the determined 3' cDNA sequence for J1-13 SEQ ID NO: 15 is the determined 5' cDNA sequence for J1-13 SEQ ID NO: 16 is the determined 3' cDNA sequence for J1-19 SEQ ID NO: 17 is the determined 5' cDNA sequence for J1-19 SEQ ID NO: 18 is the determined 3' cDNA sequence for J1-25 SEQ ID NO: 19 is the determined 5' cDNA sequence for J1-25 SEQ ID NO: 20 is the determined 5' cDNA sequence for J1-24 SEQ ID NO: 21 is the determined 3' cDNA sequence for J1-24 SEQ ID NO: 22 is the determined 5' cDNA sequence for K1-58 SEQ ID NO: 23 is the determined 3' cDNA sequence for K1-58 SEQ ID NO: 24 is the determined 5' cDNA sequence for K1-63 SEQ ID NO: 25 is the determined 3' cDNA sequence for K1-63 SEQ ID NO: 26 is the determined 5' cDNA sequence for L1-4 SEQ ID NO: 27 is the determined 3' cDNA sequence for L1-4 SEQ ID NO: 28 is the determined 5' cDNA sequence for L1-14 SEQ ID NO: 29 is the determined 3' cDNA sequence for L-14 SEQ ID NO: 30 is the determined 3' cDNA sequence for J1-12 SEQ ID NO: 31 is the determined 3' cDNA sequence for J1-16 SEQ ID NO: 32 is the determined 3' cDNA sequence for J1-21 SEQ ID NO: 33 is the determined 3' cDNA sequence for K1-48 SEQ ID NO: 34 is the determined 3' cDNA sequence for K1-55 SEQ ID NO: 35 is the determined 3' cDNA sequence for L1-2 SEQ ID NO: 36 is the determined 3' cDNA sequence for L1-6 SEQ ID NO: 37 is the determined 3' cDNA sequence for N1-1858 SEQ ID NO: 38 is the determined 3' cDNA sequence for N1-1860 SEQ ID NO: 39 is the determined 3' cDNA sequence for N-1-1861 SEQ ID NO: 40 is the determined 3' cDNA sequence for N1-1864 SEQ ID NO: 41 is the determined cDNA sequence for P5 SEQ ID NO: 42 is the determined cDNA sequence for P8 SEQ ID NO: 43 is the determined cDNA sequence for P9 SEQ ID NO: 44 is the determined cDNA sequence for P18 SEQ ID NO: 45 is the determined cDNA sequence for P20 SEQ ID NO: 46 is the determined cDNA sequence for P29 SEQ ID NO: 47 is the determined cDNA sequence for P30 SEQ ID NO: 48 is the determined cDNA sequence for P34 SEQ ID NO: 49 is the determined cDNA sequence for P36 SEQ ID NO: 50 is the determined cDNA sequence for P38 SEQ ID NO: 51 is the determined cDNA sequence for P39 SEQ ID NO: 52 is the determined cDNA sequence for P42 SEQ ID NO: 53 is the determined cDNA sequence for P47 SEQ ID NO: 54 is the determined cDNA sequence for P49 SEQ ID NO: 55 is the determined cDNA sequence for P50 SEQ ID NO: 56 is the determined cDNA sequence for P53 SEQ ID NO: 57 is the determined cDNA sequence for P55 SEQ ID NO: 58 is the determined cDNA sequence for P60 SEQ ID NO: 59 is the determined cDNA sequence for P64 SEQ ID NO: 60 is the determined cDNA sequence for P65 SEQ ID NO: 61 is the determined cDNA sequence for P73 SEQ ID NO: 62 is the determined cDNA sequence for P75 SEQ ID NO: 63 is the determined cDNA sequence for P76 SEQ ID NO: 64 is the determined cDNA sequence for P79 SEQ ID NO: 65 is the determined cDNA sequence for P84 SEQ ID NO: 66 is the determined cDNA sequence for P68 SEQ ID NO: 67 is the determined cDNA sequence for P80 (also referred to as P704P) SEQ ID NO: 68 is the determined cDNA sequence for P82 SEQ ID NO: 69 is the determined cDNA sequence for U1-3064 SEQ ID NO: 70 is the determined cDNA sequence for U1-3065 SEQ ID NO: 71 is the determined cDNA sequence for V1-3692 SEQ ID NO: 72 is the determined cDNA sequence for 1A-3905 SEQ ID NO: 73 is the determined cDNA sequence for V1-3686 SEQ ID NO: 74 is the determined cDNA sequence for R1-2330 SEQ ID NO: 75 is the determined cDNA sequence for 1B-3976 SEQ ID NO: 76 is the determined cDNA sequence for V1-3679 SEQ ID NO: 77 is the determined cDNA sequence for 1G-4736 SEQ ID NO: 78 is the determined cDNA sequence for 1G-4738 SEQ ID NO: 79 is the determined cDNA sequence for 1G-4741 SEQ ID NO: 80 is the determined cDNA sequence for 1G-4744 SEQ ID NO: 81 is the determined cDNA sequence for 1G-4734 SEQ ID NO: 82 is the determined cDNA sequence for 1H-4774 SEQ ID NO: 83 is the determined cDNA sequence for 1H-4781 SEQ ID NO: 84 is the determined cDNA sequence for 1H-4785 SEQ ID NO: 85 is the determined cDNA sequence for 1H-4787 SEQ ID NO: 86 is the determined cDNA sequence for 1H-4796 SEQ ID NO: 87 is the determined cDNA sequence for 1I-4807 SEQ ID NO: 88 is the determined cDNA sequence for 1I-4810 SEQ ID NO: 89 is the determined cDNA sequence for 1I-4811 SEQ ID NO: 90 is the determined cDNA sequence for 1J-4876 SEQ ID NO: 91 is the determined cDNA sequence for 1K-4884 SEQ ID NO: 92 is the determined cDNA sequence for 1K-4896 SEQ ID NO: 93 is the determined cDNA sequence for 1G-4761 SEQ ID NO: 94 is the determined cDNA sequence for 1G-4762 SEQ ID NO: 95 is the determined cDNA sequence for 1H-4766 SEQ ID NO: 96 is the determined cDNA sequence for 1H-4770 SEQ ID NO: 97 is the determined cDNA sequence for 1H-4771 SEQ ID NO: 98 is the determined cDNA sequence for 1H-4772 SEQ ID NO: 99 is the determined cDNA sequence for 1D-4297 SEQ ID NO: 100 is the determined cDNA sequence for 1D-4309 SEQ ID NO: 101 is the determined cDNA sequence for 1D.1-4278 SEQ ID NO: 102 is the determined cDNA sequence for 1D-4288 SEQ ID NO: 103 is the determined cDNA sequence for 1D-4283 SEQ ID NO: 104 is the determined cDNA sequence for 1D-4304 SEQ ID NO: 105 is the determined cDNA sequence for 1D-4296 SEQ ID NO: 106 is the determined cDNA sequence for 1D-4280 SEQ ID NO: 107 is the determined full length cDNA sequence for F1-12 (also referred to as P504S) SEQ ID NO: 108 is the predicted amino acid sequence for F1-12 SEQ ID NO: 109 is the determined full length cDNA sequence for J1-17 SEQ ID NO: 110 is the determined full length cDNA sequence for L1-12 (also referred to as P501S) SEQ ID NO: 111 is the determined full length cDNA sequence for N1-1862 (also referred to as P503S) SEQ ID NO: 112 is the predicted amino acid sequence for J1-17 SEQ ID NO: 113 is the predicted amino acid sequence for L1-12 (also referred to as P501S) SEQ ID NO: 114 is the predicted amino acid sequence for N1-1862 (also referred to as P503S) SEQ ID NO: 115 is the determined cDNA sequence for P89 SEQ ID NO: 116 is the determined cDNA sequence for P90 SEQ ID NO: 117 is the determined cDNA sequence for P92 SEQ ID NO: 118 is the determined cDNA sequence for P95 SEQ ID NO: 119 is the determined cDNA sequence for P98 SEQ ID NO: 120 is the determined cDNA sequence for P102 SEQ ID NO: 121 is the determined cDNA sequence for P110 SEQ ID NO: 122 is the determined cDNA sequence for P111 SEQ ID NO: 123 is the determined cDNA sequence for P114 SEQ ID NO: 124 is the determined cDNA sequence for P115 SEQ ID NO: 125 is the determined cDNA sequence for P116 SEQ ID NO: 126 is the determined cDNA sequence for P124 SEQ ID NO: 127 is the determined cDNA sequence for P126 SEQ ID NO: 128 is the determined cDNA sequence for P130 SEQ ID NO: 129 is the determined cDNA sequence for P133 SEQ ID NO: 130 is the determined cDNA sequence for P138 SEQ ID NO: 131 is the determined cDNA sequence for P143 SEQ ID NO: 132 is the determined cDNA sequence for P151 SEQ ID NO: 133 is the determined cDNA sequence for P156 SEQ ID NO: 134 is the determined cDNA sequence for P157 SEQ ID NO: 135 is the determined cDNA sequence for P166 SEQ ID NO: 136 is the determined cDNA sequence for P176 SEQ ID NO: 137 is the determined cDNA sequence for P178 SEQ ID NO: 138 is the determined cDNA sequence for P179 SEQ ID NO: 139 is the determined cDNA sequence for P185 SEQ ID NO: 140 is the determined cDNA sequence for P192 SEQ ID NO: 141 is the determined cDNA sequence for P201 SEQ ID NO: 142 is the determined cDNA sequence for P204 SEQ ID NO: 143 is the determined cDNA sequence for P208 SEQ ID NO: 144 is the determined cDNA sequence for P211 SEQ ID NO: 145 is the determined cDNA sequence for P213 SEQ ID NO: 146 is the determined cDNA sequence for P219 SEQ ID NO: 147 is the determined cDNA sequence for P237 SEQ ID NO: 148 is the determined cDNA sequence for P239 SEQ ID NO: 149 is the determined cDNA sequence for P248 SEQ ID NO: 150 is the determined cDNA sequence for P251 SEQ ID NO: 151 is the determined cDNA sequence for P255 SEQ ID NO: 152 is the determined cDNA sequence for P256 SEQ ID NO: 153 is the determined cDNA sequence for P259 SEQ ID NO: 154 is the determined cDNA sequence for P260 SEQ ID NO: 155 is the determined cDNA sequence for P263 SEQ ID NO: 156 is the determined cDNA sequence for P264 SEQ ID NO: 157 is the determined cDNA sequence for P266 SEQ ID NO: 158 is the determined cDNA sequence for P270 SEQ ID NO: 159 is the determined cDNA sequence for P272 SEQ ID NO: 160 is the determined cDNA sequence for P278 SEQ ID NO: 161 is the determined cDNA sequence for P105 SEQ ID NO: 162 is the determined cDNA sequence for P107 SEQ ID NO: 163 is the determined cDNA sequence for P137 SEQ ID NO: 164 is the determined cDNA sequence for P194 SEQ ID NO: 165 is the determined cDNA sequence for P195 SEQ ID NO: 166 is the determined cDNA sequence for P196 SEQ ID NO: 167 is the determined cDNA sequence for P220 SEQ ID NO: 168 is the determined cDNA sequence for P234 SEQ ID NO: 169 is the determined cDNA sequence for P235 SEQ ID NO: 170 is the determined cDNA sequence for P243 SEQ ID NO: 171 is the determined cDNA sequence for P703P-DE1 SEQ ID NO: 172 is the predicted amino acid sequence for P703P-DE1 SEQ ID NO: 173 is the determined cDNA sequence for P703P-DE2 SEQ ID NO: 174 is the determined cDNA sequence for P703P-DE6 SEQ ID NO: 175 is the determined cDNA sequence for P703P-DE13 SEQ ID NO: 176 is the predicted amino acid sequence for P703P-DE13 SEQ ID NO: 177 is the determined cDNA sequence for P703P-DE14 SEQ ID NO: 178 is the predicted amino acid sequence for P703P-DE14 SEQ ID NO: 179 is the determined extended cDNA sequence for 1G-4736 SEQ ID NO: 180 is the determined extended cDNA sequence for 1G-4738 SEQ ID NO: 181 is the determined extended cDNA sequence for 1G-4741 SEQ ID NO: 182 is the determined extended cDNA sequence for 1G-4744 SEQ ID NO: 183 is the determined extended cDNA sequence for 1H-4774 SEQ ID NO: 184 is the determined extended cDNA sequence for 1H-4781 SEQ ID NO: 185 is the determined extended cDNA sequence for 1H-4785 SEQ ID NO: 186 is the determined extended cDNA sequence for 1H-4787 SEQ ID NO: 187 is the determined extended cDNA sequence for 1H-4796 SEQ ID NO: 188 is the determined extended cDNA sequence for 11-4807 SEQ ID NO: 189 is the determined 3' cDNA sequence for 11-4810 SEQ ID NO: 190 is the determined 3' cDNA sequence for 11-4811 SEQ ID NO: 191 is the determined extended cDNA sequence for 1J-4876 SEQ ID NO: 192 is the determined extended cDNA sequence for 1K-4884 SEQ ID NO: 193 is the determined extended cDNA sequence for 1K-4896 SEQ ID NO: 194 is the determined extended cDNA sequence for 1G-4761 SEQ ID NO: 195 is the determined extended cDNA sequence for 1G-4762 SEQ ID NO: 196 is the determined extended cDNA sequence for 1H-4766 SEQ ID NO: 197 is the determined 3' cDNA sequence for 1H-4770 SEQ ID NO: 198 is the determined 3' cDNA sequence for 1H-4771 SEQ ID NO: 199 is the determined extended cDNA sequence for 1H-4772 SEQ ID NO: 200 is the determined extended cDNA sequence for 1D-4309 SEQ ID NO: 201 is the determined extended cDNA sequence for 1D.1-4278 SEQ ID NO: 202 is the determined extended cDNA sequence for 1D-4288 SEQ ID NO: 203 is the determined extended cDNA sequence for 1D-4283 SEQ ID NO: 204 is the determined extended cDNA sequence for 1D-4304 SEQ ID NO: 205 is the determined extended cDNA sequence for 1D-4296 SEQ ID NO: 206 is the determined extended cDNA sequence for 1D-4280 SEQ ID NO: 207 is the determined cDNA sequence for 10-d8fwd SEQ ID NO: 208 is the determined cDNA sequence for 10-H10con SEQ ID NO: 209 is the determined cDNA sequence for 11-C8rev SEQ ID NO: 210 is the determined cDNA sequence for 7.g6fwd SEQ ID NO: 211 is the determined cDNA sequence for 7.g6rev SEQ ID NO: 212 is the determined cDNA sequence for 8-b5fwd SEQ ID NO: 213 is the determined cDNA sequence for 8-b5rev SEQ ID NO: 214 is the determined cDNA sequence for 8-b6fwd SEQ ID NO: 215 is the determined cDNA sequence for 8-b6 rev SEQ ID NO: 216 is the determined cDNA sequence for 8-d4fwd SEQ ID NO: 217 is the determined cDNA sequence for 8-d9rev SEQ ID NO: 218 is the determined cDNA sequence for 8-g3fwd SEQ ID NO: 219 is the determined cDNA sequence for 8-g3rev SEQ ID NO: 220 is the determined cDNA sequence for 8-h11rev SEQ ID NO: 221 is the determined cDNA sequence for g-f12fwd SEQ ID NO: 222 is the determined cDNA sequence for g-f3rev SEQ ID NO: 223 is the determined cDNA sequence for P509S SEQ ID NO: 224 is the determined cDNA sequence for P510S SEQ ID NO: 225 is the determined cDNA sequence for P703DE5 SEQ ID NO: 226 is the determined cDNA sequence for 9-A11 SEQ ID NO: 227 is the determined cDNA sequence for 8-C6 SEQ ID NO: 228 is the determined cDNA sequence for 8-H7 SEQ ID NO: 229 is the determined cDNA sequence for JPTPN13 SEQ ID NO: 230 is the determined cDNA sequence for JPTPN14 SEQ ID NO: 231 is the determined cDNA sequence for JPTPN23 SEQ ID NO: 232 is the determined cDNA sequence for JPTPN24 SEQ ID NO: 233 is the determined cDNA sequence for JPTPN25 SEQ ID NO: 234 is the determined cDNA sequence for JPTPN30 SEQ ID NO: 235 is the determined cDNA sequence for JPTPN34 SEQ ID NO: 236 is the determined cDNA sequence for PTPN35 SEQ ID NO: 237 is the determined cDNA sequence for JPTPN36 SEQ ID NO: 238 is the determined cDNA sequence for JPTPN38 SEQ ID NO: 239 is the determined cDNA sequence for JPTPN39 SEQ ID NO: 240 is the determined cDNA sequence for JPTPN40 SEQ ID NO: 241 is the determined cDNA sequence for JPTPN41 SEQ ID NO: 242 is the determined cDNA sequence for JPTPN42

SEQ ID NO: 243 is the determined cDNA sequence for JPTPN45 SEQ ID NO: 244 is the determined cDNA sequence for JPTPN46 SEQ ID NO: 245 is the determined cDNA sequence for JPTPN51 SEQ ID NO: 246 is the determined cDNA sequence for JPTPN56 SEQ ID NO: 247 is the determined cDNA sequence for PTPN64 SEQ ID NO: 248 is the determined cDNA sequence for JPTPN65 SEQ ID NO: 249 is the determined cDNA sequence for JPTPN67 SEQ ID NO: 250 is the determined cDNA sequence for JPTPN76 SEQ ID NO: 251 is the determined cDNA sequence for JPTPN84 SEQ ID NO: 252 is the determined cDNA sequence for JPTPN85 SEQ ID NO: 253 is the determined cDNA sequence for JPTPN86 SEQ ID NO: 254 is the determined cDNA sequence for JPTPN87 SEQ ID NO: 255 is the determined cDNA sequence for JPTPN88 SEQ ID NO: 256 is the determined cDNA sequence for JP1F1 SEQ ID NO: 257 is the determined cDNA sequence for JP1F2 SEQ ID NO: 258 is the determined cDNA sequence for JP1C2 SEQ ID NO: 259 is the determined cDNA sequence for JP1B1 SEQ ID NO: 260 is the determined cDNA sequence for JP1B2 SEQ ID NO: 261 is the determined cDNA sequence for JP1D3 SEQ ID NO: 262 is the determined cDNA sequence for JP1A4 SEQ ID NO: 263 is the determined cDNA sequence for JP1F5 SEQ ID NO: 264 is the determined cDNA sequence for JP1E6 SEQ ID NO: 265 is the determined cDNA sequence for JP1D6 SEQ ID NO: 266 is the determined cDNA sequence for JP1B5 SEQ ID NO: 267 is the determined cDNA sequence for JP1A6 SEQ ID NO: 268 is the determined cDNA sequence for JP1E8 SEQ ID NO: 269 is the determined cDNA sequence for JP1D7 SEQ ID NO: 270 is the determined cDNA sequence for JP1D9 SEQ ID NO: 271 is the determined cDNA sequence for JP1C10 SEQ ID NO: 272 is the determined cDNA sequence for JP1A9 SEQ ID NO: 273 is the determined cDNA sequence for JP1F12 SEQ ID NO: 274 is the determined cDNA sequence for JP1E12 SEQ ID NO: 275 is the determined cDNA sequence for JP1D11 SEQ ID NO: 276 is the determined cDNA sequence for JP1C11 SEQ ID NO: 277 is the determined cDNA sequence for JP1C12 SEQ ID NO: 278 is the determined cDNA sequence for JP1B 12 SEQ ID NO: 279 is the determined cDNA sequence for JP1A12 SEQ ID NO: 280 is the determined cDNA sequence for JP8G2 SEQ ID NO: 281 is the determined cDNA sequence for JP8H1 SEQ ID NO: 282 is the determined cDNA sequence for JP8H2 SEQ ID NO: 283 is the determined cDNA sequence for JP8A3 SEQ ID NO: 284 is the determined cDNA sequence for JP8A4 SEQ ID NO: 285 is the determined cDNA sequence for JP8C3 SEQ ID NO: 286 is the determined cDNA sequence for JP8G4 SEQ ID NO: 287 is the determined cDNA sequence for JP8B6 SEQ ID NO: 288 is the determined cDNA sequence for JP8D6 SEQ ID NO: 289 is the determined cDNA sequence for JP8F5 SEQ ID NO: 290 is the determined cDNA sequence for JP8A8 SEQ ID NO: 291 is the determined cDNA sequence for JP8C7 SEQ ID NO: 292 is the determined cDNA sequence for JP8D7 SEQ ID NO: 293 is the determined cDNA sequence for P8D8 SEQ ID NO: 294 is the determined cDNA sequence for JP8E7 SEQ ID NO: 295 is the determined cDNA sequence for JP8F8 SEQ ID NO: 296 is the determined cDNA sequence for JP8G8 SEQ ID NO: 297 is the determined cDNA sequence for JP8B 10 SEQ ID NO: 298 is the determined cDNA sequence for JP8C10 SEQ ID NO: 299 is the determined cDNA sequence for JP8E9 SEQ ID NO: 300 is the determined cDNA sequence for JP8E10 SEQ ID NO: 301 is the determined cDNA sequence for JP8F9 SEQ ID NO: 302 is the determined cDNA sequence for JP8H9 SEQ ID NO: 303 is the determined cDNA sequence for JP8C12 SEQ ID NO: 304 is the determined cDNA sequence for JP8E11 SEQ ID NO: 305 is the determined cDNA sequence for JP8E12 SEQ ID NO: 306 is the amino acid sequence for the peptide PS2#12 SEQ ID NO: 307 is the determined cDNA sequence for P71P SEQ ID NO: 308 is the determined cDNA sequence for P712P SEQ ID NO: 309 is the determined cDNA sequence for CLONE23 SEQ ID NO: 310 is the determined cDNA sequence for P774P SEQ ID NO: 311 is the determined cDNA sequence for P775P SEQ ID NO: 312 is the determined cDNA sequence for P715P SEQ ID NO: 313 is the determined cDNA sequence for P710P SEQ ID NO: 314 is the determined cDNA sequence for P767P SEQ ID NO: 315 is the determined cDNA sequence for P768P SEQ ID NO: 316325 are the determined cDNA sequences of previously isolated genes SEQ ID NO: 326 is the determined cDNA sequence for P703PDE5 SEQ ID NO: 327 is the predicted amino acid sequence for P703PDE5 SEQ ID NO: 328 is the determined cDNA sequence for P703P6.26 SEQ ID NO: 329 is the predicted amino acid sequence for P703P6.26 SEQ ID NO: 330 is the determined cDNA sequence for P703PX-23 SEQ ID NO: 331 is the predicted amino acid sequence for P703PX-23 SEQ ID NO: 332 is the determined full length cDNA sequence for P509S SEQ ID NO: 333 is the determined extended cDNA sequence for P707P (also referred to as 11-C9) SEQ ID NO: 334 is the determined cDNA sequence for P714P SEQ ID NO: 335 is the determined cDNA sequence for P705P (also referred to as 9-F3) SEQ ID NO: 336 is the predicted amino acid sequence for P705P SEQ ID NO: 337 is the amino acid sequence of the peptide P1S#10 SEQ ID NO: 338 is the amino acid sequence of the peptide p5 SEQ ID NO: 339 is the predicted amino acid sequence of P509S SEQ ID NO: 340 is the determined cDNA sequence for P778P SEQ ID NO: 341 is the determined cDNA sequence for P786P SEQ ID NO: 342 is the determined cDNA sequence for P789P SEQ ID NO: 343 is the determined cDNA sequence for a clone showing homology to Homo sapiens MM46 mRNA SEQ ID NO: 344 is the determined cDNA sequence for a clone showing homology to Homo sapiens TNF-alpha stimulated ABC protein (ABC50) mRNA SEQ ID NO: 345 is the determined cDNA sequence for a clone showing homology to Homo sapiens mRNA for E-cadherin SEQ ID NO: 346 is the determined cDNA sequence for a clone showing homology to Human nuclear-encoded mitochondrial serine hydroxymethyltransferase (SHMT) SEQ ID NO: 347 is the determined cDNA sequence for a clone showing homology to Homo sapiens natural resistance-associated macrophage protein (NRAMP2) SEQ ID NO: 348 is the determined cDNA sequence for a clone showing homology to Homo sapiens phosphoglucomutase-related protein (PGMRP) SEQ ID NO: 349 is the determined cDNA sequence for a clone showing homology to Human mRNA for proteosome subunit p40 SEQ ID NO: 350 is the determined cDNA sequence for P777P SEQ ID NO: 351 is the determined cDNA sequence for P779P SEQ ID NO: 352 is the determined cDNA sequence for P790P SEQ ID NO: 353 is the determined cDNA sequence for P784P SEQ ID NO: 354 is the determined cDNA sequence for P776P SEQ ID NO: 355 is the determined cDNA sequence for P780P SEQ ID NO: 356 is the determined cDNA sequence for P544S SEQ ID NO: 357 is the determined cDNA sequence for P745S SEQ ID NO: 358 is the determined cDNA sequence for P782P SEQ ID NO: 359 is the determined cDNA sequence for P783P SEQ ID NO: 360 is the determined cDNA sequence for unknown 17984 SEQ ID NO: 361 is the determined cDNA sequence for P787P SEQ ID NO: 362 is the determined cDNA sequence for P788P SEQ ID NO: 363 is the determined cDNA sequence for unknown 17994 SEQ ID NO: 364 is the determined cDNA sequence for P781P SEQ ID NO: 365 is the determined cDNA sequence for P785P SEQ ID NO: 366-375 are the determined cDNA sequences for splice variants of B305D. SEQ ID NO: 376 is the predicted amino acid sequence encoded by the sequence of SEQ ID NO: 366. SEQ ID NO: 377 is the predicted amino acid sequence encoded by the sequence of SEQ ID NO: 372. SEQ ID NO: 378 is the predicted amino acid sequence encoded by the sequence of SEQ ID NO: 373. SEQ ID NO: 379 is the predicted amino acid sequence encoded by the sequence of SEQ ID NO: 374. SEQ ID NO: 380 is the predicted amino acid sequence encoded by the sequence of SEQ ID NO: 375. SEQ ID NO: 381 is the determined cDNA sequence for B716P. SEQ ID NO: 382 is the determined full-length cDNA sequence for P711P. SEQ ID NO: 383 is the predicted amino acid sequence for P711P. SEQ ID NO: 384 is the cDNA sequence for P1000C. SEQ ID NO: 385 is the cDNA sequence for CGI-82. SEQ ID NO:386 is the cDNA sequence for 23320. SEQ ID NO:387 is the cDNA sequence for CGI-69. SEQ ID NO:388 is the cDNA sequence for L-iditol-2-dehydrogenase. SEQ ID NO:389 is the cDNA sequence for 23379. SEQ ID NO:390 is the cDNA sequence for 23381. SEQ ID NO:391 is the cDNA sequence for KIAA0122. SEQ ID NO:392 is the cDNA sequence for 23399. SEQ ID NO:393 is the cDNA sequence for a previously identified gene. SEQ ID NO:394 is the cDNA sequence for HCLBP. SEQ ID NO:395 is the cDNA sequence for transglutaminase. SEQ ID NO:396 is the cDNA sequence for a previously identified gene. SEQ ID NO:397 is the cDNA sequence for PAP. SEQ ID NO:398 is the cDNA sequence for Ets transcription factor PDEF. SEQ ID NO:399 is the cDNA sequence for HTGR. SEQ ID NO:400 is the cDNA sequence for KIAA0295. SEQ ID NO:401 is the cDNA sequence for 22545. SEQ ID NO:402 is the cDNA sequence for 22547. SEQ ID NO:403 is the cDNA sequence for 22548. SEQ ID NO:404 is the cDNA sequence for 22550. SEQ ID NO:405 is the cDNA sequence for