Implantable prosthetic or tissue expanding device Number:6,849,092 from the United States Patent and Trademark Office (PTO) owispatent A keratin hydrogel-filled implantable prosthetic device. One device is a breast implant for augmenting or reconstructing a human breast including an envelope containing a keratin hydrogel. One keratin hydrogel is formed from a solid precursor which forms a keratin hydrogel upon addition of water. One source of keratin is human hair. In one method, an envelope suitable for implantation and a solid keratin hydrogel precursor are provided. The solid can be in fibrous or powder form. The solid precursor can be inserted into the envelope interior. A small incision near the breast can be made and the envelope inserted into the incision. After insertion, water can be injected into the envelope interior, preferably through the incision and through a self-sealing port in the envelope. In one method, the implant is provided as a kit, with the envelope and keratin hydrogel provided. The hydrogel can be injected into the envelope either before or after insertion into the breast area. One kit has a powdered, keratin hydrogel precursor disposed within the envelope interior, awaiting the addition of water, preferably after insertion of the implant into the body. Implantable, prosthetic, Implantable, pro, 6849092.html, Patent, pto, 6849092

Title: Implantable prosthetic or tissue expanding device

Abstract: A keratin hydrogel-filled implantable prosthetic device. One device is a breast implant for augmenting or reconstructing a human breast including an envelope containing a keratin hydrogel. One keratin hydrogel is formed from a solid precursor which forms a keratin hydrogel upon addition of water. One source of keratin is human hair. In one method, an envelope suitable for implantation and a solid keratin hydrogel precursor are provided. The solid can be in fibrous or powder form. The solid precursor can be inserted into the envelope interior. A small incision near the breast can be made and the envelope inserted into the incision. After insertion, water can be injected into the envelope interior, preferably through the incision and through a self-sealing port in the envelope. In one method, the implant is provided as a kit, with the envelope and keratin hydrogel provided. The hydrogel can be injected into the envelope either before or after insertion into the breast area. One kit has a powdered, keratin hydrogel precursor disposed within the envelope interior, awaiting the addition of water, preferably after insertion of the implant into the body.

Patent Number: 6,849,092 Issued on 02/01/2005 to Van Dyke, et al.

Inventors: Van Dyke; Mark E. (Fair Oaks Ranch, TX); Blanchard; Cheryl R. (Warsaw, IN); Timmons; Scott F. (San Antonio, TX); Siller-Jackson; Arlene J. (Helotes, TX); Smith; Robert A. (Jackson, MS)

Assignee: Keraplast Technologies, Ltd. (San Antonio, TX)

Appl. No.: 802113

Filed: March 8, 2001

Current U.S. Class: 623/17.12; 623/17.16

Intern'l Class: A61F 002/44

Field of Search: 623/17.11-17.16

References Cited [Referenced By]

U.S. Patent Documents

922692	May., 1909	Goldsmith.
926999	Jul., 1909	Neuberg.
960914	Jun., 1910	Heinemann.
1214299	Jan., 1917	Grosvenor et al.
2434688	Jan., 1948	Evans	18/47.
2445028	Jul., 1948	Jones et al.	106/155.
2517572	Aug., 1950	Jones et al.	106/155.
2814851	Dec., 1957	Hervey	28/82.
3033755	May., 1962	Jacobi et al.	167/90.
3642498	Feb., 1972	Anker	99/166.
3655416	Apr., 1972	Vinson et al.	106/155.
3867728	Feb., 1975	Stubstad et al.	3/1.
3875595	Apr., 1975	Froning	3/1.
3987497	Oct., 1976	Stoy et al.	3/1.
4021382	May., 1977	Stoy et al.	260/2.
4026296	May., 1977	Stoy et al.	128/349.
4060081	Nov., 1977	Yannas et al.	128/156.
4107121	Aug., 1978	Stoy	260/29.
4123406	Oct., 1978	Stoy et al.	260/29.
4135942	Jan., 1979	Kikkawa	106/155.
4172823	Oct., 1979	Stoy et al.	260/29.
4178361	Dec., 1979	Cohen et al.	424/22.
4183884	Jan., 1980	Wichterle et al.	264/41.
4228056	Oct., 1980	Stoy	260/29.
4309777	Jan., 1982	Patil	3/1.
4349921	Sep., 1982	Kuntz	3/1.
4357274	Nov., 1982	Werner	260/123.
4369037	Jan., 1983	Matsunaga et al.	8/127.
4423032	Dec., 1983	Abe et al.	424/70.
4439417	Mar., 1984	Matsunaga et al.	424/70.
4485097	Nov., 1984	Bell	424/95.
4495173	Jan., 1985	Matsunaga et al.	424/70.
4570629	Feb., 1986	Widra	128/156.
4685447	Aug., 1987	Iversen et al.	128/1.
4714469	Dec., 1987	Kenna	623/17.
4751074	Jun., 1988	Matsunaga et al.	424/70.
4766005	Aug., 1988	Montgomery et al.	427/4.
4772287	Sep., 1988	Ray et al.	623/17.
4837379	Jun., 1989	Weinberg	424/101.
4839168	Jun., 1989	Abe et al.	424/74.
4895722	Jan., 1990	Abe et al.	424/71.
4904260	Feb., 1990	Ray et al.	623/17.
4904261	Feb., 1990	Dove et al.	623/17.
4911718	Mar., 1990	Lee et al.	623/17.
4932969	Jun., 1990	Frey et al.	623/17.
4936848	Jun., 1990	Bagby	623/17.
4946378	Aug., 1990	Hirayama et al.	623/17.
4959213	Sep., 1990	Brod et al.	514/21.
4969888	Nov., 1990	Scholten et al.	606/94.
5035716	Jul., 1991	Downey	623/17.
5047055	Sep., 1991	Bao et al.	623/17.
5047249	Sep., 1991	Rothman et al.	424/543.
5059193	Oct., 1991	Kuslich	606/61.
5123926	Jun., 1992	Pisharodi	623/17.
5134031	Jul., 1992	Kagechi et al.	428/373.
5192326	Mar., 1993	Bao et al.	623/17.
5258043	Nov., 1993	Stone	623/66.
5276138	Jan., 1994	Yamada et al.	530/357.
5292362	Mar., 1994	Bass et al.	106/124.
5304378	Apr., 1994	Koga et al.	424/445.
5306309	Apr., 1994	Wagner et al.	623/17.
5320796	Jun., 1994	Harashima et al.	264/349.
5324775	Jun., 1994	Rhee et al.	525/54.
5358935	Oct., 1994	Smith et al.	514/21.
5390683	Feb., 1995	Pisharodi	128/898.
5443514	Aug., 1995	Steffee	623/17.
5458643	Oct., 1995	Oka et al.	623/18.
5474770	Dec., 1995	Broly et al.	424/94.
5480430	Jan., 1996	Carlisle et al.	623/8.
5505732	Apr., 1996	Michelson	606/61.
5514180	May., 1996	Heggeness et al.	623/17.
5522898	Jun., 1996	Bao	623/17.
5534028	Jul., 1996	Bao et al.	623/17.
5534029	Jul., 1996	Shima	623/17.
5549672	Aug., 1996	Maddock et al.	623/8.
5549679	Aug., 1996	Kuslich	623/17.
5552452	Sep., 1996	Khadem et al.	522/63.
5561107	Oct., 1996	Jaynes et al.	514/12.
5562736	Oct., 1996	Ray et al.	623/17.
5589451	Dec., 1996	Wilson	512/2.
5632774	May., 1997	Babian	623/8.
5634945	Jun., 1997	Pernia et al.	623/11.
5645597	Jul., 1997	Krapiva	623/17.
5653984	Aug., 1997	Fodor et al.	424/195.
5660857	Aug., 1997	Haynes et al.	424/450.
5674295	Oct., 1997	Ray et al.	623/17.
5676702	Oct., 1997	Ratron	623/17.
5679819	Oct., 1997	Jones et al.	556/418.
5694946	Dec., 1997	Tenerz et al.	128/748.
5702455	Dec., 1997	Saggar	623/17.
5703047	Dec., 1997	Wilson	514/12.
5705780	Jan., 1998	Bao	204/157.
5707805	Jan., 1998	Rubin et al.	435/6.
5712252	Jan., 1998	Smith	514/21.
5716404	Feb., 1998	Vacanti et al.	623/8.
5716415	Feb., 1998	Steffee	623/17.
5723331	Mar., 1998	Tubo et al.	435/366.
5725582	Mar., 1998	Bevan et al.	623/17.
5728405	Mar., 1998	McDonnell	424/682.
5755814	May., 1998	Berg et al.	623/66.
5763583	Jun., 1998	Arai et al.	530/353.
5766252	Jun., 1998	Henry et al.	623/17.
5782831	Jul., 1998	Sherman et al.	606/61.
5785964	Jul., 1998	Naughton et al.	424/93.
5786217	Jul., 1998	Tubo et al.	435/402.
5791352	Aug., 1998	Reich et al.	128/898.
5792090	Aug., 1998	Ladin	602/48.
5800549	Sep., 1998	Bao et al.	623/17.
5814605	Sep., 1998	Pierce et al.	514/12.
5824093	Oct., 1998	Ray et al.	623/17.
5824113	Oct., 1998	Hogo	8/128.
5824331	Oct., 1998	Usala	424/424.
5827328	Oct., 1998	Buttermann	623/17.
5842477	Dec., 1998	Naughton et al.	128/898.
5854207	Dec., 1998	Lee et al.	514/2.
5865846	Feb., 1999	Bryan et al.	623/17.
5874500	Feb., 1999	Rhee et al.	525/54.
5888224	Mar., 1999	Beckers et al.	623/17.
5914265	Jun., 1999	Roop et al.	435/320.
5916565	Jun., 1999	Rose et al.	424/195.
5916870	Jun., 1999	Lee et al.	514/2.
5932552	Aug., 1999	Blanchard et al.	514/21.
5948428	Sep., 1999	Lee et al.	424/426.
5948432	Sep., 1999	Timmons et al.	424/443.
5955083	Sep., 1999	Bonte et al.	424/195.
5972031	Oct., 1999	Biedermann et al.	623/17.
5976186	Nov., 1999	Bao et al.	623/17.
6004323	Dec., 1999	Park et al.	606/61.
6008013	Dec., 1999	Reynolds	435/69.
6022376	Feb., 2000	Assell et al.	623/17.
6063061	May., 2000	Wallace et al.	604/181.
6063378	May., 2000	Nohara et al.	424/94.
6077987	Jun., 2000	Breitbart et al.	623/11.
6093205	Jul., 2000	McLeod et al.	623/17.
6105581	Aug., 2000	Eggers et al.	128/898.
6110208	Aug., 2000	Soranzo et al.	623/15.
6110210	Aug., 2000	Norton et al.	623/17.
6110487	Aug., 2000	Timmons et al.	424/443.
6110891	Aug., 2000	Pusztai et al.	514/8.
6113639	Sep., 2000	Ray et al.	623/11.
6124265	Sep., 2000	Timmons et al.	514/21.
6124273	Sep., 2000	Drohan et al.	514/55.
6274163	Aug., 2001	Blanchard et al.	424/443.
6306177	Oct., 2001	Felt et al.	623/23.
Foreign Patent Documents
1056986	Dec., 1991	CN.
531446	Jan., 1941	DE.
3922203	Oct., 1990	DE.
4222763	Jan., 1994	DE.
0454600	Oct., 1991	EP.
0 454 600	Oct., 1991	EP.
0468797	Dec., 1995	EP.
531446	Jan., 1941	GB.
S55-187190	Dec., 1980	JP.
56129035	Oct., 1981	JP.
SHO 54-124043	Feb., 1982	JP.
SHO59-155248	Sep., 1984	JP.
SHO 60-220068	Nov., 1985	JP.
1988-202582	Aug., 1988	JP.
03011099	Jan., 1991	JP.
04091138	Mar., 1992	JP.
04091138	Mar., 1992	JP.
1992-174659	May., 1992	JP.
HEI 4-189833	Jul., 1992	JP.
5285374	Nov., 1993	JP.
5285375	Nov., 1993	JP.
1993285374	Nov., 1993	JP.
1993285375	Nov., 1993	JP.
6116300	Apr., 1994	JP.
1994100600	Apr., 1994	JP.
1994116300	Apr., 1994	JP.
HEI 6-293631	Oct., 1994	JP.
06336499	Dec., 1994	JP.
6336499	Dec., 1994	JP.
1998291999	Nov., 1998	JP.
1998337466	Dec., 1998	JP.
2001087754	Apr., 2001	JP.
WO 91/02538	Mar., 1991	WO.
WO 98/08550	Mar., 1998	WO.
WO 99/26595	Jun., 1999	WO.
WO 03/011894	Feb., 2003	WO.
WO 03/018673	Mar., 2003	WO.

Other References

Wake, et al., "Dynamics of Fibrovascular Tissue Ingrowth in Hydrogel Foams," Cell Transplantation 4(3), 275-79, 1995.
Southwest Research Institute Annual Report, 17-18, 21, 1997.
Plant, et al., "Axonal growth within poly (2-hydroxyethyl methacrylate) sponges infiltrated with Schwann cells and implanted into the lesioned rat optic tract," Brain Research, 671, 119-30, 1995.
Technology Today, 16(3):9, 1995.
Rhodes, et al., "Characteristics of the alpha-keratose fraction of hair inducing ascosporogensis in Nannizia grubyia," Mycopathologia et Mycologia Applicata, 33, 345-48, 1967.
Chargaff, et al., "Techniques for the demonstration by chromatography of nitrogenous lipide constituents, sulfur-containing amino acids, and reducing sugars," J. Biol. Chem., 175(1):67-71, 1948.
Yamauchi, et al., "Cultivation of fibroblast cells on keratin-coated substrata," Polymers for Tissue Engineering, 329-40, 1998.
Thomas et al., "Isolation of microfibrillar proteins of wool in disulfide form," Melliand Texiberichte, 65(3):20809, 1984.
van de Locht, "Reconstitution of microfibrils from wool and filaments from epidermis proteins," Melliand Textiberichte, 10:780-6, 1987.
Yoshioka et al., "Cosmetic base," unexamined Japanese Patent Application No. 3-223207, Oct. 2, 1991.
Yoshioka et al., "Water-soluble hair dressing agent," unexamined Japanese Patent Application No. 8-157342, Jun. 18, 1996.
Hyuku et al., "Novel amino acid silicone polymer, production thereof, cosmetic particles surface treated with the polymer, and cosmetic containing said particles," unexamined Japanese Patent Application No. 2001-114647, Apr. 24, 2001.
Ito et al., "Biocompatibility of denatured wool keratin," 39:4, 249-256, Apr. 1982.
Yamauchi, "The development of keratin: characteristics of polymer films," Fragrance J, 21(5), 62-7, 1993.
Sauk et al., "Reconstitution of cytokeratin filaments in vitro: further evidence for the role of nonhelical peptides in filament assembly," The Journal of Cell Biology, 99, 1590-1597, Nov. 1984.
Weber et al., "The structural relation between intermediate filament proteins in living cells and the .alpha.-keratins of sheep wool," The EMBO Journal, 1:10, 1155-1160, 1982.
Hanukoglu et al., "The cDNA sequence of a human epidermal keratin: divergence of sequence but conservation of structure among intermediate filament proteins," Cell, 31, 243-252, Nov. 1982.
Fraser et al., "Intermediate filaments in .alpha.-keratins," Proc. Natl. Acad. Sci. USA, 83, 1179-1183, Mar. 1986.
Jones, "Studies on microfibrils from .alpha.-keratin," Biochimica et Biophysica Acta, 446, 515-524, Received Apr. 5th, 1976.
Zackroff, et al., "In vitro assembly of intermediate filaments from baby hamster kidney (BHK-21) cells," Proc. Natl. Acad. Sci. USA, 76:12, 6226-6230, Dec. 1979.
Mack, et al., "Solid-state NMR studies of the dynamics and structure of mouse keratin intermediate filaments," Biochemistry, 27, 5418-5426, 1988.
Skerrow, et al., "Epidermal .alpha.-keratin is neutral-buffer-soluble and forms intermediate filaments under physiological conditions in vitro," Biochimica et Biophysica, 915, 125-131, 1987.
Kvedar, et al., "Cytokeratins of the bovine hoof: classification and studies on expression," Biochimica et Biophysica Acta, 884, 462-473, 1986.
Moll, et al., "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells," Cell, 31, 11-24, Nov. 1982.
Iwatsuki, et al., "Comparative studies on naturally occurring antikeratin antibodies in human sera," The Journal of Investigative Dermatology, 87:2, 179-184, Aug. 1986.
Lambre, et al., "An enzyme immunoassay for auto-antibodies to keratin in normal human serum and in pleural fluids from patients with various malignant or non-malignant lung diseases," J. Clin. Lab. Immunol., 20, 171-176, 1986.
Stokes, et al., "Passage of water and electrolytes through natural and artificial keratin membranes," Desalination, 42, 321-328, 1982.
Dedeurwaerder, et al., "Selective extraction of a protein fraction from wool keratin," Nature, 265, 48-49, and 274-276, Jan. 20, 1977.
Brunner, et al., "Fractionation of tyrosine-rich proteins from oxidized wool by ion-exchange chromatography and preparative electrophoresis," Eur. J. Biochem., 32, 350-355, 1973.
Mies, et al., "Chromatographic and electrophoretic investigations of the properties of unprotected low-sulphur wool kerateins," Journal of Chromatography, 405, 365-370, 1987.
Katsuumi, et al., "Two-dimensional electrophoretic analysis of human hair keratins, especially hair matrix proteins," Arch. Dermatol. Res., 281, 495-501, 1989.
Horn, et al., "Relative molecular masses of reduced wool keratin polypeptides," Biochem Soc Trans, 14, 333-334, 1986.
Harrap, et al., "Species differences in the proteins of feathers," Comp. Biochem. Physiol., 20, 449-460, 1967.
Harrap, et al., "Soluble derivatives of feather keratin," Biochem. J., 92, 8-18, 1964.
Yoshimizu, et al., ".sup.13 C CP/MAS NMR study of the conformation of stretched or heated low-sulfur keratin protein films," Macromolecules, 24, 862-866, 1991.
Schaller, et al., "Membranes prepared from keratin-polyacrylonitrile graft copolymers," Journal of Applied Polymer Science, 25, 783-794, 1980.
Weiss, et al., "The use of monoclonal antibody to keratin in human epidermal disease: alterations in immunohistochemical staining pattern," The Journal of Investigative Dermatology, 81, 224-230, 1983.
Starger, et al., "Biochemical and immunological analysis of rapidly purified 10-nm filaments from baby hamster kidney (BHK-21) cells," J. Cell Biology, 78, 93-109, 1978.
Noishiki, et al., "Application of denatured wool keratin derivatives to an antithrombogenic biomaterial--vascular graft coated with a heparinized keratin derivative--," Inst. Thermal Spring Res. Okayama Univ., 39:4, 221-227, 1982.
Dale, "Keratin and other coatings for pills," Pharm. J., 129, 494-495, 1932, Abstract.
Schrooyen, et al., "Biodegradable films from selectively modified feather keratin dispersions," Polymer Preprints (American Chemical Society, Division of Polymer Chemistry), 39(2), 160, 1998, Abstract.
Schrooyen, et al., "Polymer films from chicken feather keratin," Book of Abstracts, 216th ACS National Meeting, Boston, Aug. 23-27, 1998, Abstract.
Kikkawa, et al., "Solubilization of keratin. 6. Solubilization of feather keratin by oxidation with performic acid," Hikaku Kagaku, 20(3), 151-162, 1974, Abstract.
Matsunaga, et al., "Studies on the chemical property of human hair keratin. Part 1. Fractionation and amino acid composition of human hair keratin solubilized by performic acid oxidation," Hikaku Kagaku, 27(1), 21-29, 1981, Abstract.
Noishiki et al., "Application of denatured wool keratin derivatives to an antithrombogenic biomaterial. Vascular graft coated with a heparinized keratin derivative," Kobunshi Ronbunshu, 39(4), 221-227, 1982, Abstract.
Ito, et al., "Biocompatibility of denatured keratins from wool," Kobunshi Ronbunshu, 39(4), 249-256, 1982, Abstract.
Gillespie,, et al., "Amino acid composition of a sulphur-rich protein from wool," Biochimica et Biophysica Acta, 39, 538-539, 1960.
Gough, et al., "Amino acid sequences of .alpha.-helical segments from S-carboxymethylkerateine-A. Complete sequence of a type-I segment," Biochem. J., 173, 373-385, 1978.
Elleman, et al., "Amino acid sequences of .alpha.-helical segments from S-carboxymethylkerateine-A. Statistical analysis," Biochem. J., 173, 387-391, 1978.
Hogg, et al., "Amino acid sequences of .alpha.-helical segments from S-carboxymethylkerateine-A. Tryptic and chymotryptic peptides from type-II segment," Biochem. J., 173, 353-363, 1978.
Earland, et al., "Studies on the structure of keratin. II. The amino acid content of fractions isolated from oxidized wool," Biochemica et Biophysica Acta, 22, 405-411, 1956.
Crewther, et al., "Amino acid sequences of .alpha.-helical segments from S-carboxymethylkerateine-A. Complete sequence of a type-II segment," Biochem. J., 173, 365-371, 1978.
Fraser, et al., "Microscopic observations of the alkaline-thioglycollate extraction of wool," Biochimica et Biophysica Acta, 22, 484-485, 1953.
Gillespie, et al., "Preparation of an electrophoretically homogeneous keratin derivative from wool," Biochimica et Biophysica Acta, 12, 481-483, 1953.
Blagrove, et al., "The electrophoresis of the high-tyrosine proteins of keratins on cellulose acetate strips," Comp. Biochem. Physiol., 50B, 571-572, 1975.
Frenkel, et al., "The isolation and properties of a tyrosine-rich protein from wool: component 0.62," Eur. J. Biochem., 34, 112-119, 1973.
Marshall, et al., "Successful isoelectric focusing of wool low-sulphur proteins," Journal of Chromatography, 172, 351-356, 1979.
Marshall, "Characterization of the proteins of human hair and nail by electrophoresis," The Journal of Investigative Dermatology, 80:6, 519-524, 1983.
Lindley, et al., "Occurrence of the cys-cys sequence in keratins," J. Mol. Biol., 30, 63-67, 1967.
Marshall, "Genetic variation in the proteins of human nail," The Journal of Investigative Dermatology, 75:3, 264-269, 1980.
Goddard, et al., "A study on keratin," J. Bio. Chem., 106, 605-614, 1934.
Dowling, et al., "Isolation of components from the low-sulphur proteins of wool by fractional precipitation," Preparative Biochemistry, 4(3), 203-226, 1974.
Crewther, et al., "Reduction of S-carboxymethylcysteine and methionine with sodium in liquid ammonia," Biochimica et Biophysica Acta, 194, 606-609, 1969.
Gillespie, "The isolation from wool of a readily extractable protein of low sulphur content," Biochimica et Biophysica Acta, 27, 225-226, 1958.
Lindley, et al., "The reactivity of the disulphide bonds of wool," Biochem. J., 139, 515-523, 1974.
Mitsui, et al., "Genes for a range of growth factors and cyclin-dependent kinase inhibitors are expressed by isolated human hair follicles," British Journal of Dermatology, 137(5), 693-698, 1997, Abstract.
Schornig, et al., "Synthesis of nerve growth factor mRNA in cultures of developing mouse whisker pad, a peripheral target tissue of sensory trigeminal neurons," The Journal of Cell Biology, 120:6, 1471-1479, 1993.
Filshie, et al., "The fine structure of .alpha.-keratin," J. Mol. Biol., 3, 784-786, 1961.
Filshie, et al., "An electron microscope study of the fine structure of feather keratin," The Journal of Cell Biology, 13, 1-2, 1962.
Crewther, et al., "Low-sulfur proteins from .alpha.-keratins. Interrelationships between their amino acid compositions, .alpha.-helix contents, and the supercontraction of the parent keratin," Biopolymers, 4, 905-916, 1966.
Bhatnagar, et al., "The conformance of the high-sulphur proteins of wool. I. The preparation and properties of a water-soluble metakeratin," Int. J. Protein Research I, 199-212, 1969.
Crewther, et al., "The preparation and properties of a helix-rich fraction obtained by partial proteolysis of low sulfur S-carboxymethylkerateine from wool," The Journal of Biological Chemistry, 242:19, 4310-4319, 1967.
Parry, et al., "Structure of .alpha.-keratin: structural implication of the amino acid sequences of the type I and type II chain segments," J. Mol. Biol., 113, 449-454, 1977.
Suzuki, et al., "X-ray diffraction and infrared studies of an .alpha.-helical fragment from .alpha.-keratin," J. Mol. Biol., 73, 275-278, 1973.
Bhatnagar, et al., "The conformation of the high-sulphur proteins of wool. II. Difference spectra of kerateine-B," Int. J. Protein Research I, 213-219, 1969.
Steinert, et al., "In vitro studies on the synthesis of guinea pig hair keratin proteins," Biochimica et Biophysica Acta, 312, 403-412, 1973.
Rogers, "Some observations on the proteins of

Free Web Sudoku Puzzles.
Solve with your browser.

2 1 4 5 6
4 7 2
9 3
3 2 7
1 6
8 5 1
1 8
2 6 3
4 2 3 8 9

What is it?