Senior Fitness - Exercise and Nutrition for Aging Men and Women
FREE Article Feed for your website.
Home Ownership Magazine
Party Planning Information
Article Marketing Resources
Bio-Medical Research Article Database
Informative Articles on Life, Love and Happiness
Tutorials on Business to Writing
Famous Quotes from Famous People
Song Lyric Information
New US Patent Information
Comprehensive List of Content by Category
Online Auctions and Shopping Related Articles
Article Search
Most Recent Articles
Title: Optical disk drive and correcting method for differential push-pull tracking error signals of the optical disk drive
Patent Number: 7,436,739 Issued on 10/14/2008 to Ou Yang

Title: Play-back device
Patent Number: 7,436,738 Issued on 10/14/2008 to Yamasaki,   et al.

Title: Electric timepiece
Patent Number: 7,436,737 Issued on 10/14/2008 to Fukuda,   et al.

Title: Hydrophone array module
Patent Number: 7,436,736 Issued on 10/14/2008 to Schneider,   et al.

Title: Method for seismic processing, in particular for compensating birefringence on seismic traces
Patent Number: 7,436,735 Issued on 10/14/2008 to Garotta

Title: Shaped high frequency vibratory source
Patent Number: 7,436,734 Issued on 10/14/2008 to Krohn

Title: System for performing read operation on non-volatile storage with compensation for coupling
Patent Number: 7,436,733 Issued on 10/14/2008 to Mokhlesi

Title: Internal power supply generating circuit without a dead band
Patent Number: 7,436,732 Issued on 10/14/2008 to Hirobe

Title: Semiconductor device and method for driving the same
Patent Number: 7,436,731 Issued on 10/14/2008 to Iwata

Title: Method and device for controlling internal power voltage, and semiconductor memory device having the same
Patent Number: 7,436,730 Issued on 10/14/2008 to Cho

Title: Fuse circuit and semiconductor device using fuse circuit thereof
Patent Number: 7,436,729 Issued on 10/14/2008 to Yoshida,   et al.

Title: Fast random access DRAM management method including a method of comparing the address and suspending and storing requests
Patent Number: 7,436,728 Issued on 10/14/2008 to Harrand,   et al.

Title: Method and apparatus to control a power consumption of a memory device
Patent Number: 7,436,727 Issued on 10/14/2008 to Jain,   et al.

Title: Circuit for and method of reading data in an asynchronous FIFO including a backup address circuit for re-reading data
Patent Number: 7,436,726 Issued on 10/14/2008 to Lovejoy

Title: Data generator having stable duration from trigger arrival to data output start
Patent Number: 7,436,725 Issued on 10/14/2008 to Miki

Title: Method and system for independent control of voltage and its temperature co-efficient in non-volatile memory devices
Patent Number: 7,436,724 Issued on 10/14/2008 to Nandi

Title: Method for two-cycle sensing in a two-terminal memory array having leakage current
Patent Number: 7,436,723 Issued on 10/14/2008 to Rinerson,   et al.

Title: Semiconductor device
Patent Number: 7,436,722 Issued on 10/14/2008 to Mizuno,   et al.

Title: Supplying voltage to a bit line of a memory device
Patent Number: 7,436,721 Issued on 10/14/2008 to Lehmann,   et al.

Title: Semiconductor memory device
Patent Number: 7,436,720 Issued on 10/14/2008 to Nobutoki,   et al.

Title: Auto-precharge signal generating circuit
Patent Number: 7,436,719 Issued on 10/14/2008 to Yun

Title: Semiconductor memory device including fuse detection circuit to determine successful fuse-cutting rate for optical fuse-cutting conditions
Patent Number: 7,436,718 Issued on 10/14/2008 to Tanaka,   et al.

Title: Semiconductor device having mechanism capable of high-speed operation
Patent Number: 7,436,717 Issued on 10/14/2008 to Hidaka

Title: Nonvolatile memory
Patent Number: 7,436,716 Issued on 10/14/2008 to Kishi,   et al.

Title: Non-volatile memory device, and control method of non-volatile memory device
Patent Number: 7,436,715 Issued on 10/14/2008 to Kato,   et al.

Title: Nonvolatile semiconductor memory
Patent Number: 7,436,714 Issued on 10/14/2008 to Yaegashi,   et al.

Title: Reducing the impact of program disturb
Patent Number: 7,436,713 Issued on 10/14/2008 to Hemink

Title: Nonvolatile memory device including circuit formed of thin film transistors
Patent Number: 7,436,712 Issued on 10/14/2008 to Ogura,   et al.

Title: Semiconductor memory device
Patent Number: 7,436,711 Issued on 10/14/2008 to Cho,   et al.

Title: EEPROM memory device with cell having NMOS in a P pocket as a control gate, PMOS program/erase transistor, and PMOS access transistor in a common well
Patent Number: 7,436,710 Issued on 10/14/2008 to Ratnakumar,   et al.

Title: NAND flash memory with boosting
Patent Number: 7,436,709 Issued on 10/14/2008 to Higashitani

Title: NAND memory device column charging
Patent Number: 7,436,708 Issued on 10/14/2008 to Roohparvar

Title: Flash memory cell structure and operating method thereof
Patent Number: 7,436,707 Issued on 10/14/2008 to Hung,   et al.

Title: Method and apparatus for varying the programming duration and/or voltage of an electrically floating body transistor, and memory cell array implementing same
Patent Number: 7,436,706 Issued on 10/14/2008 to Popoff,   et al.

Title: Multiple level cell memory device with single bit per cell, re-mappable memory block
Patent Number: 7,436,705 Issued on 10/14/2008 to Roohparvar

Title: Non-volatile memory devices and method thereof
Patent Number: 7,436,704 Issued on 10/14/2008 to Kim,   et al.

Title: Active boosting to minimize capacitive coupling effect between adjacent gates of flash memory devices
Patent Number: 7,436,703 Issued on 10/14/2008 to Pham,   et al.

Title: Integrated circuit with a data memory protected against UV erasure
Patent Number: 7,436,702 Issued on 10/14/2008 to Naura,   et al.

Title: Single poly EPROM device with double control gates to prevent unintentionally charging/discharging
Patent Number: 7,436,701 Issued on 10/14/2008 to Oberhuber

Title: MRAM memory cell having a weak intrinsic anisotropic storage layer and method of producing the same
Patent Number: 7,436,700 Issued on 10/14/2008 to Ruehrig,   et al.

Title: Nonvolatile semiconductor memory device
Patent Number: 7,436,699 Issued on 10/14/2008 to Tanizaki,   et al.

Title: MRAM arrays and methods for writing and reading magnetic memory devices
Patent Number: 7,436,698 Issued on 10/14/2008 to Lin,   et al.

Title: Memory cell, memory using the memory cell, memory cell manufacturing method, and memory recording/reading method
Patent Number: 7,436,697 Issued on 10/14/2008 to Murakami,   et al.

Title: Phase-change semiconductor memory device and method of programming the same
Patent Number: 7,436,693 Issued on 10/14/2008 to Kang,   et al.

Title: Phase change memory cell with junction selector and manufacturing method thereof
Patent Number: 7,436,692 Issued on 10/14/2008 to Pellizzer,   et al.

Title: Electronic device
Patent Number: 7,436,677 Issued on 10/14/2008 to Noritsune,   et al.

Title: Heat-dissipating assembly of computer housing
Patent Number: 7,436,665 Issued on 10/14/2008 to Chen,   et al.

Title: Electronic appliance
Patent Number: 7,436,664 Issued on 10/14/2008 to Hori,   et al.

Title: Mounting for dual hard drives
Patent Number: 7,436,658 Issued on 10/14/2008 to Haager,   et al.

Title: Personal computing device with removable accessory module
Patent Number: 7,436,655 Issued on 10/14/2008 to Homer,   et al.

Title: Circuit and method for filtering a radio frequency signal
Patent Number: 7,436,249 Issued on 10/14/2008 to Hjelm

Title: Wet cleaning process and wet cleaning equipment
Patent Number: 6,866,723 Issued on 03/15/2005 to Ueda,   et al.

Title: Iso-reflectance wavelengths
Patent Number: 7,120,553 Issued on 10/10/2006 to Benvegnu

Title: Process for calibration of a medical-technical device and a medical-technical device
Patent Number: 7,120,547 Issued on 10/10/2006 to Herrmann,   et al.

Title: Polyethylene protective yarn
Patent Number: 6,764,764 Issued on 07/20/2004 to Tam,   et al.

Title: Chemical aggregation process using inline mixer
Patent Number: 6,764,802 Issued on 07/20/2004 to Maric,   et al.

Title: Method and apparatus for configurable hardware augmented program generation
Patent Number: 6,876,214 Issued on 04/05/2005 to Crook,   et al.

Title: Heterologous G protein coupled receptors expressed in yeast, their fusion with G proteins and use thereof in bioassay
Patent Number: 7,148,053 Issued on 12/12/2006 to Pausch,   et al.

Title: 4-formylamino-N-methylpiperidine derivatives, the use thereof as stabilizers and organic material stabilized therewith
Patent Number: 6,737,527 Issued on 05/18/2004 to Haremza,   et al.

Title: Metal alloys for the reflective or the semi-reflective layer of an optical storage medium
Patent Number: 6,764,735 Issued on 07/20/2004 to Nee

Title: Multi-element electron-transfer optical detector system
Patent Number: 6,765,190 Issued on 07/20/2004 to Jordan

Title: Trenched gate non-volatile semiconductor method with the source/drain regions spaced from the trench by sidewall dopings
Patent Number: 6,764,904 Issued on 07/20/2004 to Liu,   et al.

Title: Magnetic resonance imaging coated assembly
Patent Number: 6,765,144 Issued on 07/20/2004 to Wang,   et al.

Title: Multichip module having chips on two sides
Patent Number: 6,765,152 Issued on 07/20/2004 to Giri,   et al.

Title: Method of producing information recording medium, production apparatus and information recording medium
Patent Number: 6,764,737 Issued on 07/20/2004 to Arakawa,   et al.

Title: Over-coating composition for photoresist, and processes for forming photoresist patterns using the same
Patent Number: 6,764,806 Issued on 07/20/2004 to Jung,   et al.

Title: One's complement cryptographic combiner
Patent Number: 6,760,440 Issued on 07/06/2004 to Driscoll

Title: Plasma deposited selective wetting material
Patent Number: 6,764,812 Issued on 07/20/2004 to Kubacki

Title: Recognizing film and video objects occuring in parallel in single television signal fields
Patent Number: 6,937,655 Issued on 08/30/2005 to De Haan,   et al.

Title: 3-D graphics chip with embedded DRAM buffers
Patent Number: 6,937,242 Issued on 08/30/2005 to Wu,   et al.

Title: Apparatus and method employed in disk drive to enable single signal line to be used for transmission of both read and control signals
Patent Number: 6,937,410 Issued on 08/30/2005 to Koyanagi

Title: Video encoder and/or decoder
Patent Number: 6,937,661 Issued on 08/30/2005 to Narita

Title: Method and apparatus for removing heat from a circuit
Patent Number: 6,937,471 Issued on 08/30/2005 to Haws,   et al.

Title: Apparatus and method for preventing an erroneous operation at the time of detection of a system failure
Patent Number: 7,117,397 Issued on 10/03/2006 to Kondo,   et al.

Title: Devices and methods for controlling movement of an electrosurgical electrode
Patent Number: 7,189,232 Issued on 03/13/2007 to Scholl,   et al.

Pharmaceutical compositions which inhibit vascular proliferation and method of use thereof Number:7,084,117 from the United States Patent and Trademark Office (PTO) owispatent

Home    Author Login    Submit Article    Article Search    Add Your Link    Edit Your Link    Contact Us    Advertising    Disclaimer

   

 
Web LinkGrinder.com

Top Breaking News
     Greek, Cypriot Leaders Resume Unification Talks in Nicosia by Nathan Morley
     Indonesia Tobacco Sales Grow, Raising Health Fears
     South Korea Allows Top Defector to Travel Overseas by VOA News

Title: Pharmaceutical compositions which inhibit vascular proliferation and method of use thereof

Abstract: The present invention relates to a method of treating vascular proliferation in a patient in need thereof. The method includes the step of administering a therapeutically effective amount of a type-1 somatostatin agonist to said patient.

Patent Number: 7,084,117 Issued on 08/01/2006 to Culler,   et al.


Inventors: Culler; Michael Dewitt (Hopkinton, MA); Danesi; Romano (Leghorn, IT); Bocci; Guido (Lucca, IT); Deltacca; Mario (Pisa, IT)
Assignee: Societe de Conseils de Recherches et d'Applications Scientifiques, SAS (Paris, FR)
Appl. No.: 10/466,152
Filed: January 14, 2002
PCT Filed: January 14, 2002
PCT No.: PCT/US02/01125
371(c)(1),(2),(4) Date: November 18, 2003
PCT Pub. No.: WO02/064160
PCT Pub. Date: August 22, 2002


Current U.S. Class: 514/11 ; 514/16; 530/311
Current International Class: A61K 38/31 (20060101)
Field of Search: 514/11,16,414 530/311 548/468


References Cited [Referenced By]

U.S. Patent Documents
5750499 May 1998 Hoeger et al.
5912247 June 1999 Jinbo et al.
6060275 May 2000 Hacohen et al.
6903074 June 2005 Morgan et al.
2002/0103526 August 2002 Steinke
Foreign Patent Documents
96/07398 Mar., 1996 WO
99/049884 Oct., 1999 WO
99/056769 Nov., 1999 WO
99/065508 Dec., 1999 WO
00/12111 Mar., 2000 WO
WO 00/10552 Mar., 2000 WO

Other References

Albini et al. Somatostatin controls Kaposi's sarcoma tumor growth through inhibition of angiogenesis. FASEB Journal. 1999, vol. 13, pp. 647-655. cited by examiner .
Antonian et al. Biotransformations Of The Anti-Angiogenic Compound SU5416. Drug Metabolism And Disposition. 2000, vol. 28, No. 12, pp. 1505-1512. cited by examiner .
Vajkoczy et al. Inhibition of Tumor Growth, Angiogenesis . . . Neoplasia. Apr. 1999, vol. 1, No. 1, pp. 31-41. cited by examiner .
Mccombe, M. et al., "Effect of a Long-acting Somatostatin Analogue (BIM23104) on Proliferative Diabetic Retinopathy: A Pilot Study," Eye, 1991, 5(5):569-575. cited by other .
Aavik, E. et al., "Elimination of Vascular Fibrointimal Hyperplasia by somatostatin receptor," FASEB, 2002, 16(7):2002-2005. cited by other .
Barrie, R. et al., "Inhibition of Angiogenisis by Somatostatin and Somatostatin-like Compounds is Structurally Dependent," J. of Surgical Research, 1993, 4(55):446-450. cited by other .
Patel, Y.C., "Somatostatin and its receptor family", Frontiers in Neuroendocrinology, 1999, 20:157-198. cited by other .
Lapinski, et al., "Development of a selective agonist at the somatostatin receptor subtype SSTR1", J. Pharm. Exper. Therap., 1996, 276:1089-1094. cited by other.

Primary Examiner: Russel; Jeffrey Edwin
Attorney, Agent or Firm: Yankwich & Associates Morrill; Brian R. Feeney; Alan F.

Parent Case Text



CROSS REFERENCE TO RELATED APPLICATIONS

This application is the National Stage of International Application No. PCT/US02/01125, filed Jan. 14, 2002, which was published in English under PCT Article 21(2), which claims benefit of United States Provisional Application Nos. 60/261,439 filed Jan. 12, 2001 and 60/275,294 filed Mar. 13, 2001.
Claims



What is claimed is:

1. A method of treating vascular proliferation in a patient, said method comprising administering a therapeutically effective amount of a somatostatin type-1 receptor agonist to said patient wherein said somatostatin type-1 receptor agonist is a somatostatin type-1 receptor selective agonist having a Ki for the somatostatin type-1 receptor which is less than 5 nM and at least 10 times less than the Ki for said somatostatin type-1 receptor agonist for each of the somatostatin type-2, type-3, type-4, and type-5 receptors provided that said somatostatin type-1 receptor agonist is not H-D-Phe-Phe-Phe-D-Trp-Lys-Thr-Phe-Thr-NH.sub.2 or 3-((2,4-dimethylpyrrol-5-yl) methylidenyl)-2-indolinone.

2. The method according to claim 1 wherein the somatostatin type-1 receptor selective agonist is Caeg-c(D-Cys-Pal-D-Trp-Lys-D-Cys)-Thr(Bzl)-Tyr-NH.sub.2 or a pharmaceutically acceptable salt thereof.

3. The method of claim 1, wherein said vascular proliferation comprises angiogenesis, restenosis, endothelial cell proliferation, smooth muscle proliferation, or new blood vessel sprouting.

4. The method of claim 1, wherein said vascular proliferation occurs in a disease or condition comprising an autoimmune disease, arthritis, scleroderma, cancerous tumors, corneal graft neovascularization, diabetic retinopathy, hemangioma, hypertrophic scarring, or psoriasis.

5. The method of claim 1, wherein said vascular proliferation is incident to or associated with angioplasty or an AV shunt.

6. The method of claim 1, wherein said vascular proliferation occurs in a disease or condition comprising warts, granulomas, Kaposi's sarcoma, or allergic oedema.

7. The method of claim 1, wherein said vascular proliferation occurs in a disease or condition comprising endometriosis, dysfunctional uterine bleeding, or follicular cysts.

8. The method of claim 1, wherein said vascular proliferation occurs in a disease or condition comprising retinopathy of prematurity, choroidopathy, macular degeneration, or age-related macular degeneration.

9. The method of claim 1, wherein said vascular proliferation occurs in a disease or condition comprising solid tumor, tumor metastasis, benign tumor, acoustic neuromas, neurofibromas, trachomas, leukemia, pyogenic granuloma, myocardial angiogenesis, plaque neovascularization, atherosclerosis, coronary collaterals, cerebral collaterals, arteriovenous malformations, ischemic limb angiogenesis, corneal graft rejection, Osler-Webber Syndrome, rubeosis, neovascular glaucoma, retrolental fibroplasia, diabetic retinopathy, diabetic neovascularization, fracture, vasculogenesis, hematopoiesis, ovulation, menstruation, placentation, cat scratch disease (Rochele minalia quintosa), peptic ulcer, Helicobacter pylori associated ulcer, psoriasis, telangiectasia psoriasis, rheumatoid arthritis, Crohn's disease, intestinal adhesions, scarring, hypertrophic scars, keloids, telangiectasia, hemophiliac joints, angiofibroma, or wound granulation.

10. The method of claim 1, wherein said somatostatin type-1 receptor agonist is disposed upon or within a vascular stent.

11. The method of claim 10, wherein said somatostatin type-1 receptor agonist is provided as a component of a slow release formulation.

12. The method of claim 10, wherein said somatostatin type-1 receptor agonist is provided as a component of a polymeric composition.
Description



BACKGROUND OF THE INVENTION

Angiogenesis, the development of new capillaries from preexisting blood vessels, is a critical process in the progression of solid neoplasms and in many other pathological conditions such as diabetic retinopathy and rheumatoid arthritis (Folkman, J., Nature Medicine 1995, 1: 27 31). Different strategies to target the vascular development have been extensively studied and the availability of reliable in vitro model systems in model systems in angiogenesis research has been crucial for the study of specific inhibitors (Jain, R. K., et al., Nature Medicine 1997, 3: 1203 1208).

It is now widely recognized that the ability of a tumor to induce proliferation of new blood vessels from its host has a profound effect on cancer growth and metastasis. The process of tumor angiogenesis is mediated by a balance of positive and negative regulators of microvessel growth and the development of new blood vessels may be divided into three different sequential steps: 1) cell-mediated, proteolytic degradation of the basement membrane; 2) endothelial cell migration and proliferation out of the vessel into the surrounding extracellular matrix; 3) organization of the cells into tube-like structures (Folkman, J., Nature Medicine 1995, 1: 27 31).

Somatostatin (somatotropin release inhibiting factor or SRIF) has both a 14 amino acid isoform (somatostatin-14) and a 28 amino acid isoform (somatostatin-28). See Wilson, J. & Foster, D., Williams Textbook of Endocrinology, p. 510 (7th ed., 1985). The compound is an inhibitor of secretion of the growth hormone and was originally isolated from the hypothalamus. Brazeau, et al., Science 179:77 (1973). Native somatostatin has a very short duration of effect in vivo since it is rapidly inactivated by endo- and exopeptidase. Many novel analogs (e.g., peptide and non-peptide compounds) have been prepared in order to enhance the duration of effect, biological activity, and selectivity (e.g., for the particular somatostatin receptor) of this hormone. Such analogs of somatostatin will be called "somatostatin agonists" herein.

Various somatostatin receptors (SSTRs) have been isolated, e.g., SSTR-1, SSTR-2, SSTR-3, SSTR-4, and SSTR-5. Thus, a somatostatin agonist may be a SSTR-1 agonist, and/or a SSTR-2 agonist, and/or a SSTR-3 agonist, and/or a SSTR-4 agonist and/or a SSTR-5 agonist.

The antiangiogenic activity of somatostatin analogues has been previously demonstrated in some in vitro and in vivo experimental models (Danesi, R. et al., Clinical Cancer Research 1997, 3: 265 272; Woltering, E. A., et al., Investigational New Drug 1997, 15: 77 86). In addition to this, long-term octreotide treatment was able to reduce the progression of neovascularization associated with severe proliferative retinopathy in diabetic patients (Mallet et al., 1992).

The determination of which somatostatin subtype or subtypes are involved in the antiangiogenic property of somatostatin would allow for the development of therapeutic compositions with maximum efficacy and minimum side effects. However, previous studies in this field have resulted in contradictory and/or inconclusive findings regarding the role which each of the five somatostatin receptor subtypes may play in respect of the antiangiogenic activity of somatostatin.

SUMMARY OF THE INVENTION

The present invention relates to compositions comprising a somatostatin type-1 receptor agonist which are useful for the inhibition of vascular proliferation in a subject. The present invention further relates to a method of treating vascular proliferation, e.g., angiogenesis and restenosis, in a patient (e.g., a mammal such as a human) in need of such treatment. The method includes the step of administering a therapeutically effective amount of a somatostatin type-1 receptor (SSTR-1) agonist (e.g., a somatostatin type-1 selective agonist) to said patient.

The present invention also relates to a method of inhibiting smooth muscle proliferation, endothelial cell proliferation, and new blood vessel sprouting in a patient in need of such inhibition. The method includes the step of administering a therapeutically effective amount of a somatostatin type-1 receptor (SSTR-1) agonist (e.g., a somatostatin type-1 selective agonist) to said patient.

Examples of clinical indications which can be treated by the present invention include, but are not limited to, autoimmune diseases (e.g., arthritis, scleroderma, etc.), cancerous tumors, corneal graft neovascularization, diabetic retinopathy, hemangioma, hypertrophic scars, and psoriasis, as well as vascular proliferation associated with surgical procedures, e.g., angioplasty and AV shunts.

Further examples of disease states that may be amenable to treatment with the subject therapeutic composition and method of the invention are, in respect of the skin: warts, granulomas, Kaposi's sarcoma, allergic oedema, and the like; in respect to the uterus and ovary: endometriosis, dysfunctional uterine bleeding, follicular cysts, and the like; in respect of the eye: retinopathy of prematurity, choroidal and other intraocular disorders, macular degeneration, age-related macular degeneration, and the like.

Further examples of disease states that may be amenable to treatment with the subject therapeutic composition and method of the invention are disclosed in Folkman, J., Seminars in Medicine of the Beth Israel Hospital, Boston, Vol. 333, No 26, pp. 1757 1763.

Indeed as is well known in the art the list of known diseases and conditions for which an agent capable of inhibiting angiogenesis, smooth muscle proliferation, endothelial cell proliferation, and/or new blood vessel sprouting is quite substantial, and includes, without limitation: solid tumors, tumor metastasis, benign tumors, for example, acoustic neuromas, neurofibromas, and trachomas, leukemia, pyogenic granuloma, myocardial angiogenesis, plaque neovascularization, atherosclerosis, coronary collaterals, cerebral collaterals, arteriovenous malformations, ischemic limb angiogenesis, ocular and corneal angiogenic conditions, for example, corneal graft rejection, Osler-Webber Syndrome, rubeosis, neovascular glaucoma, retrolental fibroplasia, and diabetic retinopathy, diabetic neovascularization, wound healing, fractures, vasculogenesis, hematopoiesis, ovulation, menstruation, placentation, cat scratch disease (Rochele minalia quintosa), ulcers (peptic; Helicobacter pylori), psoriasis, (including, e.g., telangiectasia psoriasis), rheumatoid arthritis, Crohn's disease, intestinal adhesions, scarring, (i.e., formation of high density tissue including cells and connective tissue), hypertrophic scars, (i.e., keloids), telangiectasia; hemophiliac joints; angiofibroma; and wound granulation. These diseases and conditions are discussed in detail in the literature, for example, as in the following U.S. patents and International Patent Publications.: U.S. Pat. Nos. 6,323,228, 6,294,532, 6,288,228, 6,288,024, 6,284,726, 6,280,739, 6,265,407, 6,265,403, 6,258,812, 6,255,355, 6,255,353, 6,251,867, 6,242,481, 6,235,756, 6,235,741, 6,228,879, 6,228,871, 6,225,340, 6,214,800, 6,201,104, 6,177,401, 6,174,861, 6,150,407, 6,150,362, 6,117,862, 6,114,355, 6,090,794, 6,086,865, 6,071,948, 6,057,290, 6,057,122, 6,028,061, 6,025,353, 6,025,331, 6,024,688, 6,017,949, 5,997,868, 5,994,388, 5,994,292, 5,990,280, 5,985,878, 5,985,330, 5,981,484, 5,972,922, 5,972,896, 5,948,403, 5,932,611, 5,902,790, 5,874,081, 5,847,002, 5,843,925, 5,837,680, 5,807,731, 5,801,146, 5,766,591, 5,753,230, 5,744,492, 5,733,876, 5,721,226, 5,712,291, 5,698,586, 5,696,147, 5,677,181, 5,646,136, 5,629,340, 5,629,327, 5,610,166, 5,593,990, 5,574,026, 5,567,693, 5,567,417, 5,563,130, 5,512,550, 5,506,208, WO02/02609, WO02/02593, WO02/00877, WO02/00690, WO02/00017, WO01/93806, WO01/85796, WO01/81579, WO01/81311, WO01/79157, WO01/74299, WO01/72699, WO01/72297, WO01/66127, WO01/62799, WO01/62725, WO01/59100, WO01/58899, WO01/51048, WO01/46110, WO01/45751, WO01/35977, WO01/34195, WO01/29085, WO01/28577, WO01/25433, WO01/23375, WO01/21831, WO01/19987, WO01/19868, WO01/12809, WO01/12226, WO01/12210, WO01/10859, WO01/09113, WO01/07057, WO01/04157, WO01/03720, WO01/00201, WO00/75124, WO00/73445, WO00/73340, WO00/59532, WO00/54770, WO00/54762, WO00/53757, WO00/53753, WO00/53752, WO00/52158, WO00/48591, WO00/47212, WO00/47193, WO00/43393, WO00/40597, WO00/35407, WO00/32221, WO00/32180, WO00/30628, WO00/27866, WO00/27415, WO00/27340, WO00/24415, WO00/21561, WO00/20577, WO00/20026, WO00/19995, WO00/15792, WO00/12089, WO00/10507, WO00/10506, WO00/09657, WO00/09495, WO00/05356, WO00/02902, WO00/02871, WO00/02585, WO00/01383, WO99/62549, WO99/61590, WO99/61432, WO99/60984, WO99/58139, WO99/48495, WO99/45909, WO99/37776, WO99/31088, WO99/26622, WO99/26480, WO99/23105, WO99/22760, WO99/16755, WO99/16465, WO99/14234, WO99/10349, WO99/09982, WO99/04806, WO99/04803, WO98/58929, WO98/58919, WO98/54093, WO98/51326, WO98/41205, WO98/36760, WO98/35958, WO98/31688, WO98/19712, WO98/19649, WO98/17796, WO98/13071, WO98/12226, WO98/05323, WO98/05293, WO97/45137, WO97/41824, WO97/35567, WO97/32583, WO97/30085, and WO97/26258. The contents of each of the foregoing patents and patent publications is hereby incorporated by reference in its entirety.

Definitions of "somatostatin type-1 receptor agonist" and "somatostatin type-1 receptor selective agonist" are provided below. A therapeutically effective amount depends upon the condition being treated, the route of administration chosen, and the specific activity of the compound used and ultimately will be decided by the attending physician or veterinarian (e.g., between 5 g/day and 5 mg/day). In one embodiment, the somatostatin agonist is administered to the patient until the condition being treated has subsided. In another embodiment, the somatostatin agonist is administered for the lifetime of the patient.

The somatostatin agonist may be injected parenterally, e.g., intravenously, into the bloodstream of the subject being treated. However, it will be readily appreciated by those skilled in the art that the route, such as intravenous, subcutaneous, intramuscular, intraperitoneal, enterally, transdermally, transmucously, sustained released polymer compositions (e.g., a lactic acid polymer or lactic acid and glycolic acid copolymer microparticle or implant), profusion, nasal, oral, etc., will vary with the condition being treated and the activity and bioavailability of the somatostatin agonist being used.

The somatostatin agonist may also be provided as a coating or as a component of a coating on a surface of a device implanted within the body. For example, the provision of the somatostatin agonist within or upon a vascular stent is useful for the treatment of restenosis which is often associated with stent implantation.

The SSTR-1 agonist can be administered systemically and/or locally or topically, as needed. For prevention of adhesions, the SSTR-1 agonist would typically be applied at the time of surgery, preferably in a controlled release formulation and/or using barrier technology.

While it is possible for the somatostatin agonist to be administered as the pure or substantially pure compound, it may also be presented as a pharmaceutical formulation or preparation. The formulations to be used in the present invention, for both humans and animals, comprise any of the somatostatin agonists to be described below, together with one or more pharmaceutically acceptable carriers thereof, and optionally other therapeutic ingredients.

The carrier must be "acceptable" in the sense of being compatible with the active ingredient(s) of the formulation (e.g., capable of stabilizing peptides) and not deleterious to the subject to be treated. Desirably, the formulation should not include oxidizing agents or other substances with which peptides are known to be incompatible. For example, somatostatin agonists in the cyclized form (e.g., internal cysteine disulfide bond) can be oxidized; thus, the presence of reducing agents as excipients could lead to an opening of the cysteine disulfide bridge. On the other hand, highly oxidative conditions can lead to the formation of cysteine sulfoxide and to the oxidation of tryptophane. Consequently, it is important to carefully select the excipient. pH is another key factor, and it may be necessary to buffer the product under slightly acidic conditions (pH 5 to 6).

The formulations may conveniently be presented in unit dosage form and may be prepared by any of the methods well known in the art of pharmacy. All methods include the step of bringing the active ingredient(s) into association with the carrier which constitutes one or more accessory ingredients.

In general, the formulations for tablets or powders are prepared by uniformly and intimately blending the active ingredient with finely divided solid carriers, and then, if necessary, as in the case of tablets, forming the product into the desired shape and size.

Formulations suitable for parenteral (e.g., intravenous) administration, on the other hand, conveniently comprise sterile aqueous solutions of the active ingredient(s). Preferably, the solutions are isotonic with the blood of the subject to be treated. Such formulations may be conveniently prepared by dissolving solid active ingredient(s) in water to produce an aqueous solution, and rendering said solution sterile. The formulation may be presented in unit or multi-dose containers, for example, sealed ampoules or vials.

Formulations suitable for sustained release parenteral administrations (e.g., biodegradable polymer formulations such as polyesters containing lactic or glycolic acid residues) are also well known in the art. See, e.g., U.S. Pat. Nos. 3,773,919 and 4,767,628 and PCT Publication No. WO 94/15587.

Methods and formulations for the treatment and/or coating of surgical stents, wherein said coating may comprise a pharmaceutically active compound, are also well known in the art. See, e.g., U.S. Pat. Nos. 6,214,115, 6,090,901 and 6,083,257, and International Patent Publication No.'s WO O1/01957 and WO 00/02599.

The somatostatin or somatostatin agonist may also be administered with another compound capable of lowering blood levels of triglycerides, cholesterol, or glycerol, such as fibrates (e.g., bezafibrate, gemfibrozil, and clofibrate), HMG-COA reductase inhibitors (e.g., pravastatin, simvastatin, and fluorastatin, Atorvastatin, and Lovastatin), bile acid binding resins (e.g., cholestyramine and colestipol), nicotinic acid compounds (e.g., nicotinic acid and niceritrol), and fish oils. See Workshop Treatment of Hyperlipidemia 1996 2 (Lakemedelsverket, Uppsala, Sweden, 1996).

Other features and advantages of the invention will be apparent from the following description of the preferred embodiments and from the claims.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a schematic of the treatment protocol discussed at page 13, lines 7 12;

FIG. 2 is a schematic of the treatment protocol of plancental vessel explants as discussed at page 13, line 37 to page 14, line 2;

FIG. 3 shows a view of vascular cells organized radially to form microvessels that underwent continuous remodeling;

FIGS. 4 and 5 show a framework of endothelial cells that were immunoreactive for von Willebrand factor;

FIG. 6 shows a series of microscopic pictures of placental explants on the first day of culture;

FIG. 7 is a view of a culture treated with SMS-14 10.sup.-7 M;

FIG. 8 is a view of a culture treated with BIM23014C 10.sup.-6 M;

FIG. 9 is a view of a culture treated with BIM23745C 10.sup.-8 M;

FIG. 10 is a view of a culture treated with BIM23190C 10.sup.-9 M;

FIG. 11 is a view of a culture treated with BIM23268C 10.sup.-7 M;

FIG. 12 is a view of a culture treated with BIM23206C 10.sup.-8 M;

FIG. 13 is a view of a culture treated with BIM23197C 10.sup.-7 M;

FIG. 14 is a view of a culture treated with BIM23120C 10.sup.-9 M;

FIG. 15 is a view of a culture treated with BIM23926C 10.sup.-7 M;

FIG. 16 is a view of a culture treated with SU5416 10.sup.-6 M; and

FIG 17 consists of two Tables one of which reports the results of the cytotoxicity assay (Table I) and the other reports data compiled on the activity of selective somatostatin analogues on capillaries sprouts (Table II).

DETAILED DESCRIPTION OF THE INVENTION

It is believed that one skilled in the art can, based on the description herein, utilize the present invention to its fullest extent. The following specific embodiments are, therefore, to be construed as merely illustrative, and not limitative of the remainder of the disclosure in any way whatsoever.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Also, all publications, patent applications, patents, and other references mentioned herein are incorporated by reference.

What is meant by a somatostatin type-1 receptor agonist (i.e., SSTR-1 agonist) is a compound which (1) has a high binding affinity (e.g., Ki of less than 1000 nM or preferably less than 100 nm or less than 10 nM) for SSTR-1 (e.g., as defined by the receptor binding assay described below) and (2) decreases the rate or extent of vascular proliferation, (e.g., as shown by the biological assay described below).

What is meant by a somatostatin type-1 receptor selective agonist is a somatostatin agonist which (1) has a higher binding affinity (i.e., Ki) for SSTR-1 than for either SSTR-2, SSTR-3, SSTR-4 or SSTR-5, (e.g., as defined by the receptor binding assay described below) and (2) decreases the rate or extent of vascular proliferation, (e.g., as shown by the biological assay described below).

In one embodiment, the somatostatin type-1 receptor selective agonist is also a SSTR-1 agonist.

Examples of somatostatin agonists are those covered by formulae or those specifically recited in the publications set forth below, all of which are hereby incorporated by reference.

Van Binst, G. et al. Peptide Research 5:8 (1992);

Horvath, A. et al. Abstract, "Conformations of Somatostatin Analogs Having Antitumor Activity", 22nd European peptide Symposium, Sep. 13 19, 1992, Interlaken, Switzerland;

Curtis et al., Am. J. Physiol. Heart. Circ. Physiol, 278:H1815 (2000);

Nicolaou et al., Design and synthesis of a peptidomimetic employing .beta.-D-glucose for scaffolding, in Peptides, Rivier and Marshall, eds., ESCOM (1990),

Papageorgiou et al., "Design, synthesis, and binding affinity of a non peptide mimic of somatostatin" Bioorganic & Medicinal Chemistry Letters, vol. 2, pp. 135 140, 1992; and R. Hirschmann et al. "De novo design and synthesis of somatostatin non-peptide peptidomimetics utilizing beta-D-glucose as a novel scaffolding, J. Am. Chem. Soc., vol. 115, pp. 12550 12568, 1993.

PCT Application No. WO 91/09056 (1991);

EP Application No. 0 363 589 A2 (1990);

EP Application No. P5 164 EU (Inventor: G. Keri);

U.S. Pat. No. 6,262,229

U.S. Pat. No. 6,197,963

U.S. Pat. No. 6,159,941

U.S. Pat. No. 6,127,343

U.S. Pat. No. 6,083,960

U.S. Pat. No. 6,020,349

U.S. Pat. No. 5,552,534

U.S. Pat. No. 5,817,879

U.S. Pat. No. 5,811,512

U.S. Pat. No. 4,904,642 (1990);

U.S. Pat. No. 4,871,717 (1989);

U.S. Pat. No. 4,853,371 (1989);

U.S. Pat. No. 4,725,577 (1988);

U.S. Pat. No. 4,684,620 (1987);

U.S. Pat. No. 4,650,787 (1987);

U.S. Pat. No. 4,603,120 (1986);

U.S. Pat. No. 4,585,755 (1986);

EP Application No. 0 203 031 A2 (1986);

U.S. Pat. No. 4,522,813 (1985);

U.S. Pat. No. 4,486,415 (1984);

U.S. Pat. No. 4,485,101 (1984);

U.S. Pat. No. 4,435,385 (1984);

U.S. Pat. No. 4,395,403 (1983);

U.S. Pat. No. 4,369,179 (1983);

U.S. Pat. No. 4,360,516 (1982);

U.S. Pat. No. 4,358,439 (1982);

U.S. Pat. No. 4,328,214 (1982);

U.S. Pat. No. 4,316,890 (1982);

U.S. Pat. No. 4,310,518 (1982);

U.S. Pat. No. 4,291,022 (1981);

U.S. Pat. No. 4,238,481 (1980);

U.S. Pat. No. 4,235,886 (1980);

U.S. Pat. No. 4,224,199 (1980);

U.S. Pat. No. 4,211,693 (1980);

U.S. Pat. No. 4,190,648 (1980);

U.S. Pat. No. 4,146,612 (1979);

U.S. Pat. No. 4,133,782 (1979);

U.S. Pat. No. 5,506,339 (1996);

U.S. Pat. No. 4,261,885 (1981);

U.S. Pat. No. 4,728,638 (1988);

U.S. Pat. No. 4,282,143 (1981);

U.S. Pat. No. 4,215,039 (1980);

U.S. Pat. No. 4,209,426 (1980);

U.S. Pat. No. 4,190,575 (1980);

EP Patent No. 0 389 180 (1990);

EP Application No. 0 505 680 (1982);

EP Application No. 0 083 305 (1982);

EP Application No. 0 030 920 (1980);

PCT Application No. WO 88/05052 (1988);

PCT Application No. WO 90/12811 (1990);

PCT Application No. WO 97/01579 (1997);

PCT Application No. WO 91/18016 (1991);

PCT Application No. WO 00/75186 (2000);

U.K. Application No. GB 2,095,261 (1981); and

French Application No. FR 2,522,655 (1983).

Examples of SSTR-1 selective somatostatin agonists include, but are not limited to, the following somatostatin analogs which are disclosed in the above-cited references:

H-Cys-Phe-Phe-D-Trp-Lys-Thr-Phe-Cys-NH.sub.2;

H-D-Phe-Phe-Phe-D-Trp-Lys-Thr-Phe-Thr-NH.sub.2;

H-Cys-Phe-Phe-D-Trp-Lys-Ser-Phe-Cys-NH.sub.2;

H-Cys-Phe-Tyr-D-Trp-Lys-Thr-Phe-Cys-NH.sub.2;

H-Cys-Phe-Tyr(I)-D-Trp-Lys-Thr-Phe-Cys-NH.sub.2;

##STR00001## and

Caeg-c(D-Cys-Pal-D-Trp-Lys-D-Cys)-Thr(Bzl)-Tyr-NH.sub.2, in which the structure for "Caeg" is

##STR00002##

Note that for all somatostatin agonists described herein, each amino acid residue represents the structure of --NH--C(R)H--CO--, in which R is the side chain (e.g., CH.sub.3 for Ala). Lines between amino acid residues represent peptide bonds which join the amino acids. Also, where the amino acid residue is optically active, it is the L-form configuration that is intended unless D-form is expressly designated. A disulfide bond (e.g., a disulfide bridge) exists between the two free thiols of the Cys residues; however, it is not shown.

Synthesis of Somatostatin Agonists

The methods for synthesizing somatostatin agonists is well documented and are within the ability of a person of ordinary skill in the art. For example, synthesis of H-D-Phe-Phe-Phe-D-Trp-Lys-Thr-Phe-Thr-NH.sub.2, described above, can be achieved by following the protocol set forth in Example I of European Patent Application 0 395 417 A1. The synthesis of somatostatin agonists with a substituted N-terminus can be achieved, for example, by following the protocol set forth in WO 88/02756, European Patent Application No. 0 329 295, and PCT Publication No. WO 94/04752.

Somatostatin Receptor Binding Assays

The human SSTR-1, SSTR-2, SSTR-3, SSTR-4, and SSTR-5 cDNA clones have been described (SSTR-1 and SSTR-2 in Yamada, Y., et al., Proc. Natl. Acad. Sci. USA., 89:251 255 (1992); SSTR-3 in Yamada, et al., Mol. Endocrinol. 6:2136 2142 (1993); and SSTR-4 and SSTR-5 in Yamada, et al., Biochem. Biophys. Res. Commun. 195:844 852 (1993)) and are also available from American Type Culture Collection (ATCC, Rockville, Md.) (ATCC Nos. 79044 (SSTR-1), 79046 (SSTR-2), and 79048 (SSTR-3)). Based on the restriction endonuclease maps, the entire coding region of each SSTR cDNA may be excised by suitable restriction endonuclease digestion (Maniatis, T., et al., Molecular Cloning--A Laboratory Manual, CSHL, 1982). Restriction endonucleases are available from New England Biolabs (Beverly, Mass.). This cDNA fragment was inserted into the mammalian expression vector, pCMV (Russell, D., et al., J. Biol. Chem., 264:8222 8229 (1989)), using standard molecular biology techniques (see e.g., Maniatis, T., et al., Molecular Cloning,--A Laboratory Manual, Cold Spring Harbor Laboratory, 1982) to produce the expression plasmid, pCMV-human SSTR-1 through pCMV-human SSTR-5. Other mammalian expression vectors include pcDNA1/Amp (Invitrogen, Sandlesy, Calif.). The expression plasmids were introduced into the suitable bacterial host, E. Coli HB101 (Stratagene, La Jolla, Calif.) and plasmid DNAs, for transfection, were prepared on Cesium Chloride gradients.

CHO-K1 (ovary, Chinese hamster) cells were obtained from ATCC (ATCC No. CCL 61). The cells were grown and maintained in Ham's F12 media (Gibco BRL, Grand Island, N.Y.) supplemented with 10% fetal bovine serum under standard tissue culture conditions. For transfection, the cells were seeded at a density 1.times.10.sup.6/60-cm plate (Baxter Scientific Products, McGraw Park, Ill.). DNA mediated transfection was carried out using the calcium phosphate co-precipitation method (Ausubel, F. M., et al., Current Protocols in Molecular Biology, John Wiley & Sons, 1987). The plasmid pRSV-neo (ATCC; ATCC No. 37198) was included as a selectable marker at 1/10 the concentration of the expression plasmid. CHO-K1 clonal cell lines that have stably inherited the transfected DNA were selected for growth in Ham's F12 media containing 10% fetal bovine serum and 0.5 mg/ml of G418 (Sigma). The cells were ring-cloned and expanded in the same media for analysis.

Expression of the human SSTR-1 through SSTR-5 receptors in the CHO-K1 cells were detected by Northern blot analysis of total RNA prepared from the cells (Sambrook, J. E., et al., Molecular Cloning--A Laboratory Manual, Ed. 2, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 1989) and by receptor binding using (.sup.125I-Tyr.sup.11)somatostatin-14 as a ligand. Transfected cell lines expressing the human SSTR receptors were clonally expanded in culture and used in the following SSTR binding protocol.

Crude membranes were prepared by homogenization of the transfected cells in 20 ml of ice-cold 50 mM Tris-HCl with a POLYTRON homogenizer (setting 6, 15 sec). Buffer was added to obtain a final volume of 40 ml, and the homogenate was centrifuged in a Sorval SS-34 rotor at 39,000 g for 10 min at 0 4.degree. C. The resulting supernatant was decanted and discarded. The pellet was rehomogenized in ice-cold buffer, diluted, and centrifuged as before. The final pellet was resuspended in the 10 mM Tris HCl and held on ice for the receptor binding assay.

Aliquots of the membrane preparation were incubated for 30 min at 30.degree. C. with 0.05 nM (.sup.125I-Tyr.sup.11)somatostatin-14 (2000 Ci/mmol; Amersham Corp., Arlington Heights, Ill.) in 50 mM HEPES (pH 7.4) containing a test somatostatin agonist of various concentrations (e.g., 10.sup.-11 to 10.sup.-6), 10 mg/ml bovine serum albumin (fraction V) (Sigma Chemical Co., St. Louis, Mo.), MgCl.sub.2 (5 mM), Trasylol (200 KIU ml), bacitracin (0.02 mg/ml), and phenylmethylsulphonyl fluoride (0.02 mg/ml). The final assay volume was 0.3 ml. The incubations were terminated by rapid filtration through GF/C filters (pre-soaked in 0.3% polyethylenimine for 30 min) using a Brandel filtration manifold. Each tube and filter were then washed three times with 5 ml aliquots of ice-cold buffer. Specific binding was defined as the total (.sup.125I-Tyr.sup.11)SRIF-14 bound minus that bound in the presence of 1000 nM. The Ki values for somatostatin agonists are calculated by using the following formula: Ki=IC.sub.50/(1+(LC/LEC)) where IC.sub.50 is the concentration of test somatostatin agonist required to inhibit 50 percent of the specific binding of the radioligand (.sup.125I-Tyr.sup.11)somatostatin-14, LC is the concentration of the radioligand (0.05 nM), and LEC is the equilibrium dissociation constant of the radioligand (0.16 nM).

Inhibition of Proliferation and Capillary Tube Formation

In order to investigate the antiproliferative effects on endothelial cells and the inhibition of proliferation and capillary tube formation of endothelial cells caused by somatostatin analogues, two different human in vitro models have been preformed. These models permitted study of the effects of somatostatin analogues on a bi-dimensional endothelial cell layer and on three-dimensional endothelial cell growth in an extracellular matrix that mimic the capillary development in vivo. Furthermore, these assays allowed a very long period of treatment (72 hours and 28 days, respectively) on human tissues, resembling the possible chronic clinical approach to antiangiogenic therapy.

Material and Methods

Materials

Recombinant human epidermal growth factor (EGF) and recombinant human vascular endothelial growth factor (VEGF) were from PeproTechEC LTD (London, UK). EGF and VEGF, according with the information of data sheet, were reconstituted in sterile distilled water at a concentration of 100 .mu.g/mL.

Cell culture medium 199 and medium 199 without phenol red were purchased from Gibco BRL (Paisley, UK). Type A gelatin from porcine skin supplements and all other chemicals not listed in this section were obtained from Sigma Chemical Co. (St. Louis, Mo., USA). Plastics for cell culture were supplied by Costar (Cambridge, Mass., USA).

Somatostatin-14, BIM-23014C, BIM-23120C, BIM-23190C, BIM-23197C, BIM-23206C, BIM-23268C, BIM-23745C and BIM-23926C (each from Biomeasure, Incorporated, Milford, Mass., USA) were dissolved in a stock solution of 0.01 N acetic acid containing 0.1% fatty acid-free bovine serum albumin (BSA) and stored at -80.degree. C.

The relative affinities of the foregoing compounds for the various somatostatin receptors may be summarized as follows:

TABLE-US-00001 Compound SSTR-1 SSTR-2 SSTR-3 SSTR-4 SSTR-5 Somatostatin-14 v. high v. high v. high v. high v. high BIM-23014C v. low v. high low v. low high BIM-23120C v. low v. high v. low v. low low BIM-23190C v. low v. high low v. low high BIM-23197C v. low v. high mod-high v. low high BIM-23206C v. low low v. low v. low v. high BIM-23268C high mod-high high mod-high v. high BIM-23745C mod v. low v. low v. low v. low BIM-23926C v. high v. low v. low v. low v. low

SU5416, 3-((2,4-dimethylpyrrol-5-yl) methylidenyl)-2-indolinone, was a gift from Sugen Inc. (San Francisco, Calif., USA).

Cell Culture Conditions

The immortalized human microvascular endothelial cell line HMEC-1, characterized by Ades, et al., (Journal of Investigative Dermatology 1992, 99: 683 690), was maintained in Medium 199, supplemented with 10% heat-inactivated fetal bovine serum (FBS), penicillin (50 IU/mL) and hydrocortisone (100 .mu.g/mL). Cells were routinely grown in 75 cm2 gelatin-coated tissue culture flasks and kept in a humidified atmosphere of 5% CO.sub.2 at 37.degree. C. Cells were harvested with a solution of 0.25% trypsin-0.03% EDTA when they were in long phase of growth and maintained at the above-described culture conditions for all experiments.

Cytotoxicity Assay

In vitro chemosensitivity testing was performed on single-cell suspensions of HEMC-1 cells (5.times.10.sup.3 cells/well) plated in 96-well gelatin-coated sterile plastic plates and allowed to attach overnight. The treatment protocol (FIG. 1) was designed so that after 24 hours, 10.sup.-10 10.sup.-6 M somatostatin-14, somatostatin analogues, SU5416 10.sup.-6 M+VEGF 10 ng/mL as positive control or vehicle were added, and plates were incubated for 72 hours (for more details, see FIG. 1). The treatments were scheduled so that each peptide was represented by at least nine wells. At the end of the experiment, cells were rinsed with phosphate buffered saline (PBS), harvested with trypsin/EDTA, and counted with an hemocytometer. Results are expressed as the percentage of cell proliferation versus controls and are the mean of three separate experiments.+-.S.E repeated thrice.

In vitro Cultures of Human Placental Vessels

The use of the experimental model of human placental explants, detailed below, received authorization by the Ethics committee of Pisa University Hospital (Protocol n. 005567).

The experimental procedure described by Brown et al., (Laboratory Investigation 1996, 75: 539 555) was followed and modified in the present study. Immediately after the spontaneous delivery, the placenta was collected in sterile conditions and superficial blood vessels, approximately 1 to 1.5 m in diameter and from 1 to 5 cm in length, were excised. Vessel explants were placed in phosphate buffered saline (PBS) solution containing 2.5 mg/mL of amphotericin B and 50 .mu.g/mL of gentamycin and were cut into approximately 1-mm fragments. The cultures were performed in 24-well culture plates; 0.5 mL/well of a solution of fibrinogen 3 mg/mL in Medium 199 without phenol red was added to each well followed by the quick addition of 15 .mu.L of thrombin (50 NIH U/mL in 0.15 M NaCl). The vessel explants were rapidly placed in the center of the wells after clot formation and covered by 0.5 mL/well of the fibrinogen solution with the addition of further 15 .mu.L of thrombin for suspending all of them at the same level between the two clots. After gel formation, 1 mL/well of medium 199 without phenol red supplemented with 10% of heat-inactivated FBS, 0.1% .epsilon.-aminocaproic acid, L-glutamine (2 mM), and antibiotics (streptomycin 50 .mu.g/mL, penicillin 50 IU/mL and amphotericin B 2.5 mg/mL) were added. Vessels were cultured and treated every two days with 10.sup.-10 10.sup.-6 M somatostatin-14, somatostatin analogues, and SU5416 10.sup.-6 M or vehicle at 37.degree. C. 95% air/5% CO.sub.2 in a humidified environment for 28 days (FIG. 2). Vessels explants were photographed on day 28 with a phase-contrast Leitz MD IL microscope (Leica, Heerbrugg, Switzerland) and were subjected to image analysis.

Image Analysis

The image analysis procedures described by Bocci et al., (Cancer Chemotherapy Pharmacology 1999, 43: 205 212), were adopted for the present study. Briefly, photographs obtained from the placental fragment assay were digitized in a 512.times.512-pixel matrix, using a color video camera TK-1280E (JVC, Tokyo, Japan) and a microcomputer processor. Digitized pictures were visualized on highresolution color display. The true color image analysis software package KS 300 v.1.2 (Kontron Elektronik GmbH, Eching, Germany) was run for interactive manipulation, quantification of the images and data collection. Geometric calibrations were set with a sample of known dimensions and agray-scale analysis was performed to measure the density of the image that was in the range of 0 255, where 0 was black (presence of vascular sprouts) and 255 was white (absence of vascular sprouts). In the fibrin culture of placental vessel explants the mean gray level of the sprouting area was measured and the sprouting index (SI) was defined as: Sprouting index=((sprouting area/mean gray level of sprouting area)/perimeter of explant).times.100. Results are expressed as the percentage of sprouting index+/-S.E. versus controls.

Results

Cytotoxicity Assay

All results are shown in Table I. All somatostatin analogues at the studies concentrations revealed antiproliferative activity on immortalized human microvascular endothelial HMEC-1 cells, with a maximum effect at 10.sup.-7 10.sup.-8 M. The positive control of SU5416 10.sup.-6 M, a specific VEGF-receptor inhibitor, resulted in a cell growth block of 56.2%.

In vitro Cultures of Human Placental Vessels

The explant sprouting within the fibrin matrix was characterized by numerous microvessels around the placental fragment. Vascular cells organized radially to form microvessels that underwent continuous remodeling (FIG. 3). The maximal growth of the three-dimensional microvascular network occurred during the third-fourth week and reached the plateau at 27 days after explant. Histologically, in the fibrin gel a subtle framework of endothelial cells, that was immunoreactive for von Willebrand factor (FIG. 4), was observed (FIG. 5). In most of the cases the microvessels showed an initial lumen; in other the lumen was absent and only endothelial cells were observed (FIG. 5).

The microscopic picture of the placental explants on the first day of the culture is shown in FIG. 6A (bar, 2 mm); the appearance of the outgrowth of endothelial cells from the placental cultured vessel fragment was observed approximately on the sixth day of culture (SI=0.055.+-.0.004 (mm/mean gray).times.100; FIG. 6D).

The experimental data on the activity of somatostatin analogues on capillaries sprouts were summarized in Table II. Cultures treated with SMS analogues and SU5416 were shown in FIGS. 7 16 where the maximum effects were shown. BIM-23926C and BIM-23745C revealed a potent inhibitory property in a long-term treatment; they resulted, respectively, in 17.18.+-.11.8% at 10.sup.-7 M and 42.84.+-.5.6% at 10.sup.-8 M of SI as compared to untreated controls. SU5416, the positive control, resulted in 32.92.+-.9.7% of SI at a concentration of 10.sup.-6 M.

OTHER EMBODIMENTS

The foregoing description has been limited to specific embodiments of this invention. It will be apparent, however, that variations and modifications may be made to the invention, with the attainment of some or all of the advantages of the invention. Such embodiments are also within the scope of the following claims.

*


Free Web Sudoku Puzzles.
Solve with your browser.
  1             7
      4          
      2   8 3   9
1       8     4  
    9       2    
  4     6       1
2   3 5   9      
          3      
9             8  
What is it?



Add Your Site · Terms Of Service · Privacy Policy


DISCLAIMER
Linkgrinder is a free service that searches the Internet and indexes all files found so that you may search quickly and easily for shared files. These files are created and made available individually by users whose identity we are not aware of and who we have no control over. In essence we function like a search engine tool; these files ARE NOT STORED OR SERVED BY OUR NETWORK. We are not responsible for any materials obtained by using our service. We do not monitor any of the contents of these files. These files may contain viruses, illegal materials, materials inappropriate for minors, offensive files and the like. BY USING OUR SERVICE, YOU ASSUME FULL RESPONSIBILITY FOR DOWNLOADING THESE MATERIALS AND WILL INDEMNIFY US FOR ANY DAMAGES THAT MAY BE INCURRED.

For More Specific Information VIEW OUR TERMS OF SERVICE.

Thank you and Enjoy!